FP1-WP - Wounding 0.557 mm DiameterTube Style Floating Pin, parylene coated with Blunt tip, 1 mm long tip

SKU
FP1-WP

Wounding Pin, Parylene Coated, 0.557mm diameter pin protruding 1mm from a 38mm cannula, 1.68mm in diameter, Each

Many of our customers have used our pin tools to "wound" cell culture monolayers and then study the effects of different treatments on wound healing. Initially our VP 408FH Replicator, VP 384FH Replicator or Robotic Pin Tools converted to manual use with the BMPM-PAD-1 plus a special wounding Library Copier (VP 381NW, VP 381NW4.5 or VP 381NW5) were used to make the wounds. For most applications, these wounding tools give good results. However, some customers who were using computerized image analysis programs needed the wounds to be more precisely localized. For these customers, we have developed specialized pin tools that have very tight hole tolerances in the floating fixture so the wounds are precisely and consistently located in the same position in each well. These wounding series pin tools (our “W” and “WP” pin tools) are illustrated on this page. A recent addition to our line of wounding pin tools are pins with a 0.05 mm layer of Parylene deposited using a vapor deposition process to produce a soft lining on the pin tips. Each pin now acts as “eraser” to remove the cell monolayer without scratching the plastic well surface under the monolayer. Another new innovation and problem solved only from V&P Scientific. The wounding pin tools that incorporate Parylene-coated pins can also be used in our standard floating fixtures because the pins are larger in diameter and fit snugly in the standard fixture holes. In contrast, the uncoated wounding pins need to have the smaller diameter holes found in the wounding floating fixtures in order to achieve the snug fit. Another factor that can lead to wound variation is a loose fit between the microplate and the registration device (e.g., our VP 381NW Library Copier). This is due to variation in the molds used to make the microplates. One way of dealing with the problem is to use our adjustable Library Copier (VP 381NWGV4.5). A less elegant but practical solution is to apply layers of tape to the inside of our standard wounding Library Copiers until you have a snug fit with the microplate.
More Information
Product Name FP1-WP - Wounding 0.557 mm DiameterTube Style Floating Pin, parylene coated with Blunt tip, 1 mm long tip
Description Wounding Pin, Parylene Coated, 0.557mm diameter pin protruding 1mm from a 38mm cannula, 1.68mm in diameter, Each
Pin Tool Components Pins
PIn Series FP1 Series
Pin Diameter 0.557 mm (parylene coated)
Type of Transfer Wound Healing Assays
Coating/Encapsulation Parylene C Coated
Dimensions 1.68 mm in diameter X 0.557 mm diameter Pin X 39 mm long
Pin Tip Wounding Pin

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