VP 771LE-4AS - Magnetic Separation Plate for 96 Well PCR Microplates Full Skirted, 24 Protruding Magnetic (42 MGO) Cylinders, Step Down Edge, SLAS Footprint, No Registration Base

Magnetic Bead Separation Block for 96 well PCR microplates, 24 post 42 MGO magnets, red anodized aluminum magnet frame, step down edge for full-skirted plates, SBS footprint, no Registration Base. Each
SKU: VP 771LE-4AS

Availability: In stock

 
 
  Description    Specifications    Details    Applications    Media    Blogs  
Details
Magnetic Bead Separation Block for 96 well PCR microplates, 24 post 42 MGO magnets, red anodized aluminum magnet frame, step down edge for full-skirted plates, SBS footprint, no Registration Base. Each
Additional Info
Mode of Use No
Format 96
Container To Be Used Microplates
Well Bottom Shape Pyramid Bottom, V-Bottom
Plate Type PCR
Materials - Attributes Frame - Anodized Aluminum
Number of Magnets 24 Cylinders
MGO - Attribute 42
Magnet Type - Attribute Neodymium (NdFeB)
Includes Registration Base No.
SLAS Dimensions Yes.
Dimensions No
Bead Pellet Location 1 Side, Middle
Magnetic Orientation Axial, North Poles Up
Tube Size No
Bottle Volume No
Robot Gripper Access No.

Magnetic Bead Separation Devices:


V&P has developed 82 different magnetic bead separation devices for microplates of nearly every description:

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# 4 well, 6 well, 12 well, 24 well, 48 well, 96 well, 384 well and1536 well microplates and PCR plates

# Standard height, midi height, deep well

# Round well, square well, octagon well, conical well

# Round bottom, flat bottom, pyramid bottom

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 Match the microplate to the Magnetic Bead Separation Device:


  It is important to match the right magnetic bead separation device with your particular microplate. There are many characteristics of microplates that will affect where and how tightly the magnetic beads are pelleted:

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# The thickness of the microplate well wall

#  The smoothness of the interior well

# The geometry of the well walls

# The geometry of the well bottoms

# The geometry of the skirt

# The geometry of underneath side of the microplate

# The location and size of plastic injection ports on the underneath side of the microplates  as it varies with each microplate manufacturer and affects location of magnet placement

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All of the above features are very important and we have dealt with all of them over the course of the last 10 years and that is why we say “choose your microplate wisely before you launch a new assay protocol”.  With our large selection of magnetic separation devices we can help you make the best decision for your application.  We are happy to share our knowledge with you.

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Magnetic Beads:


 We also consider the magnetic beads or particles you are using:

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# Size of the beads

# Their magnetic moment

# Their concentration

 

We may be able to suggest a specific product for your application. If speed of separation is important to you, larger magnetic bead particles with greater magnetic moment will separate faster rather than weaker smaller particles.

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Magnetic Bead Separation Location:


Depending on your protocol, you may want the beads to be collected:

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# On the side of the wells

# High up the well

# Low down the well

# Or both high and low in the same Magnetic Separation device depending on different process, elution or wash volumes   

# Directly in the bottom center of the well

# On the bottom outside ring of the well

# The mass or volume of the beads to be pelleted also determines which device is best

# The volume of supernatant and the method of removal (decanting, needle aspiration or pipetting) are also important considerations

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Magnetic Field Orientation and Strength:

 


The magnetic field orientation of adjacent magnets has a force multiplying effect (Halbach effect) and results in the magnetic beads being more tightly held:

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# We use Halbach arrays in several of our designs to improve pelleting 

# The normal magnetic field strength of the magnet also affects how tightly the beads are held

# How close the magnet is placed to the magnetic beads also affects how tightly the beads are held 

 

 

Types of Supernatant Separation - aspiration or removal:


More commonly, magnetic beads are separated by aspiration using pipet tips and then washed. By using pipet tips, it is important to keep the beads away from the pipet. This is why bead pellet location and how tightly it is magnetically held is so important.  Similar consideration also applies to needle aspiration used in automatic plate washers.

 

V&P Scientific was the first to develop the Flick and Blot system for removing supernatant solutions from magnetic bead pellets. These devices allow for the microplate to be fixed to the magnet plate. You can then pick up the entire device and "Flick" out the supernatant into a sink or other container. This is a fast, economical way of removing supernatant from your microplates while still retaining your magnetic beads in the microplate for more rounds of washing.

 

Recently V&P Scientific developed a different method to separate the supernatant from the magnetic beads by using a 96 magnetic pin replicator (VP 407AM-N) that is submerged into the wells and will remove the beads from the microplate and leave the supernatant behind in the microplate. Using a PCR plate as a shield, you can use our handheld magnetic pin tool device to move the beads from one microplate to another. This will save you time as it only takes a few seconds.  It will also save you money as no expensive pipet tips are needed, all you need is an inexpensive PCR plate. 

 

Advantages of V&P Scientific’s Magnetic Separation Devices


# Largest number of devices produced by any manufacturer

# Greatest variety of devices to fit all Microplates

# Greatest variety for pellet location

# Strongest Magnets available in many different sizes

# Custom magnetic Separation Devices not a problem

# Our specialty is Flexibility

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Accessories


See our accessories for aspirating supernatantsadding wash solutions and  for keeping magnetic beads uniformly in suspension prior to adding to microplate wells.

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Published Articles

Click here for a list of published articles that cite our Magnetic Separation Devices

 Applications of Magnetic Bead Separation Devices


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Since the introduction of Magnetic Beads as a method to separate bound from unbound ligands the applications and variation of Magnetic Bead Assays has soared exponentially, and they are literally too numerous to count.  Magnetic Bead Separation Assays have rapidly replaced the slower and more cumbersome techniques of absorption (ELISA), centrifugation and filtration separation methods.  In addition, Magnetic Bead assays have increased the recovery of sample and its purity with fewer wash steps.

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Magnetic Beads have been successfully coupled to antibodies, antigens, proteins, viruses, bacteria, fungi, Ligands, DNA, RNA, Avidin, Streptavidin, Biotin, staphylococcal protein A and the list goes on….  Furthermore, these Magnetic beads have also been directly labeled with radio isotopes, enzymes, dyes, fluorescent compounds and complementary metal oxide semiconductors (CMOS) and quantum dots (QD) for direct detection with biosensors resulting in even more capability and multiplexing opportunities. 

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This said, Magnetic Bead Separation assays are the clear and obvious method of choice for the foreseeable future.  V&P Scientific will work with developers of new Magnetic Bead assays to provide the perfect Magnetic Bead Separation device. We have already developed 82 different Magnetic Bead Separation devices for microplates and 29 for micro-tubes, test tubes and bottles.  Many of these are sold on an OEM basis to both diagnostic companies and plate washing companies.

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We have also developed devices for keeping magnetic beads in uniform suspension while being pipetted into microplates. See this link for sealed bearing suspension reservoirs and this link  for Tumble Stirrer Magnetic bead suspension reservoirs.

Gifs

None at this time