Glass Slide Arrayers

V&P Scientific is proud to introduce the first economical and practical way to make cDNA, DNA, RNA or protein microarrays on glass slides. Whether you want to make large arrays on glass slides, work out new protocols for your robotic Glass Slide Arrayer or just Q/C new DNA preparations, this simple and flexible system allows you to tackle both large and small jobs without major setup hassles. 


Convenience Versatility Enhanced Features Spot Size Spot Pitch Accessories Available Custom Options
Print your own microarrays anytime from 96 or 384 well plates Can spot your samples as duplicates, triplicates or more Print up to 768 spots per array.  Print duplicate slides at the same time. ~500 microns in diameter on silanated slides and 780 microns on Epoxy coated slides 1,125 microns on horizontal axis and 750 microns on the vertical axis, can change pitch 
easily for different spot sizes by skipping rows or columns
Indexing System for 96 and 384 well source plates to simplify arraying and enhance spot size reproducibility.  Replicator pin wash station to enhance arraying speed.

Can be adapted to your robotic system, change pins for different applications, e.g., larger volumes


Our floating Pin Replicator can be fixtured with either 2 rows of 4 pins (8 total on 9 mm centers for 96 well microplates (VP 478)

or 4 rows of 8 pins (32 total) on 4.5 mm center for 384 well microplates (VP 478A). Then used in conjunction with the unique

V&P Scientific Glass Slide Indexing System VP 470 (US Patent #6,613,284 and Republic of China Invention Patent # NI-164,388,

and European patent #1,363,737) this dual slide system places 768 spots ~500 microns in diameter, onto a grid 18 mm wide and

36 mm tall on two separate slides. 


     

The glass slide to the right illustrates the 768 spotting pattern on a silanated glass slide made by Xenopore

The glass slide to the right is an epoxy coated slide from Xenopore spotted with 5 mm 24mer oligonucleotide

with a 3' amine linker

and a 5' Cy5 label in 50% DMSO.

The GenePix 3 software was used to determine a mean spot diameter of 780 microns with a CV of 5.5%.

This is an enlarged area of the photo above.

Aldehyde (Silanated) Slide with Amino/Cy5-Labeled Oligo

768 spot array, 5' Cy5 and 3' Amino linker

Fluorescence Data from GSI

Lumonics Scan of the slide



Accuracy and Precision Data from the Slide above Spot Diameter

Position Mean SD %CV Max Min
Pin 1 793.1 42.7 5.4% 920 700
Pin 2 786.7 42.1 5.4% 910 700
Pin 3 789.2 40.5 5.1% 890 710
Pin 4 786.3 35.4 4.5% 870 930
Pin 5 787.5 39.1 5.0% 910 710
Pin 6 757.9 41.4 5.5% 870 690
Pin 7 795.8 41.7 5.2% 930 730
Pin 8 745.2 37.4 5.0% 840 690
Total 780.2 43.3 5.5% 930 690

21 Item(s)

per page

21 Item(s)

per page