Wounding Pin Tools

FP4-W Pin   

FP6-WP Pin FP6-WP Pin

Images courtesy of BioTek:

HT-1080 cells dispensed into 96-well Greiner SCREENSTAR Plates

-Cycloolefin

-190 µm thick film bottom

Following attachment, twound was created by manually scratching pins through cell layer from one side of the well to the other.

Plate placed into the Lionheart FX to capture kinetic wound healing

-Instrument setup to capture brightfield and high contrast bright field images

-Single 4x images captured with each channel every 30min for 24 hrs. 

-Gen5 cellular analysis used to quantify cell coverage within captured images.

Many of our customers have used our pin tools to "wound" cell culture monolayers to study the effects of different wound-healing treatments. Initially our VP 408FH Replicator, VP 384FH Replicator, or Robotic Pin Tools converted to manual use with the BMPM-PAD-1; using a special Wounding Library Copier (VP 381NW, VP 381NW4.5 or VP 381NW5) they were used to make the wounds. For most applications, these wounding tools give good results, however, some customers who were using computerized image analysis programs needed the wounds to be more precisely localized. For these customers, we have developed specialized pin tools that have very tight tolerances in the floating fixture so that the wounds are precisely and consistently located in the same position in each well. These wounding series pin tools (our “W” and “WP” pin tools) are illustrated on this page.

 

A recent addition to our line of wounding pin tools are pins with a 0.05 mm layer of Parylene deposited using a vapor deposition process to produce a soft lining on the pin tips.  Each pin now acts as “eraser” to remove the cell monolayer without scratching the plastic well surface under the monolayer.  Another new innovation and problem solved only from V&P Scientific.

Wounding Dimensions in Microplates

The photo to the right shows the wounding dimensions of VP 381NW,

VP 381NW4.5 and VP 381NW5 with FP,FP6, FP6-WP, FP4-WP and FP1-WP pins.
 

This is how the Small Molecule Screening Facility at Keck-UWCCC does their

quantitative wound healing assay using VP 408FH.
http://www.hts.wisc.edu/Resources.htm#wh

Also see photos of wounding pin tools on old web site  http://www.vp-scientific.com/wounding_tissue_culture_experiments.php#FP_-WP

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