As the trend towards assay miniaturization continues with increasing demands to make nanoliter transfers, here is a simple way to convert a FLIPRTETRATM into a 384 or 1,536 pin tool for UHTS.
The FLIPRTETRA system from Molecular Devices delivers an entirely new screening platform to address the real-time kinetic cell-based assays. This instrument broadens the FLIPRTETRA system’s capabilities to include 1536 simultaneous liquid transfer, multi-wavelength kinetic reading and agile internal plate handling, in addition to elevated instrument intelligence. Combined, these features enable users to save time and money, while addressing their preferred screening targets.
In particular, Molecular Devices’ FLIPRTETRA1536 pin tool liquid transfer technology significantly reduces screening time and reagent consumption. Simultaneous liquid transfer to approximately 2,000 cells per well in a 1536-well plate is achieved using a 1536 pin tool. Contact liquid transfer occurs with the same height, speed and a range of delivery volumes from 19 nL to 656 nL.
FLIPRTETRA using pins from V&P Scientific, allows direct transfer of compounds that have been suspended in 100% DMSO or ethanol. The transfer of compounds in nanoliter volumes (as low as 19 nL {or lower on a custom pin}) eliminates the need for an intermediate dilution plate to decrease DMSO concentration. Because transfer volumes are small, DMSO concentrations are 1% or less, and toxic effects to the cells are minimized. In addition, by transferring compounds directly from the library to the read plated, consumable costs, including plates and tips, are significantly decreased, minimizing assay preparation time and increasing throughput.
The pin tool also allows non-specific or “sticky” compounds to transfer easily using the optional hydrophobic-lipophobic pin coating. Because the compounds do not stick to the pins, accurate dispensing of compounds is achieved.
FLIPRTETRA provides user-configurable fluidic components to enable you to exchange pin tools with traditional pipettors in less than 10 minutes. Pin tools are available for different liquid volumes in 384 and 1536-well formats.
ScreenWorksTM software makes it easy to transition between pin tools and adjust transfer volume. ScreenWorks also enables you to customize pin washing using up to two solvents, as well as automate the use and exchange of disposable blotting stations.
