Overview
V&P Scientific is proud to introduce the first economical and practical way to make microarrays on glass slides for under $7,000.00 including accessories. Now you can make your own DNA or protein microarrays without buying a $20,000 machine. Whether you want to make large arrays on glass slides, work out new protocols for your robotic Glass Slide Arrayer or just Q/C new DNA preparations, this simple and flexible system allows you to tackle both large and small jobs without major setup hassles. Now you can have:
- Convenience
Print your own microarrays anytime from 96 or 384 well plates - Versatility
Spot your samples as duplicates, triplicates, or more - Enhanced Features
Print up to 768 spots per array. Print duplicate slides at the same time. - Spot Size
~500 microns in diameter on silanated slides and 780 microns on Epoxy coated slides - Spot Pitch
1,125 microns on the horizontal axis and 750 microns on the vertical axis, can change the pitch
easily for different spot sizes by skipping rows or columns - Accessories Available
Indexing System for 96 and 384 well source plates to simplify arraying and enhance spot size reproducibility. Replicator pin wash station to enhance arraying speed. - Custom Options
Can be adapted to your robotic system, change pins for different applications, e.g., larger volumes
Our Floating Pin Replicator can be fixtured with either 2 rows of 4 pins (8 total) on 9 mm centers for 96 well microplates (VP 478) see photo #1 or 4 rows of 8 pins (32 total) on 4.5 mm centers for 384 well microplates (VP 478A). When used in conjunction with the unique V&P Scientific Glass Slide Indexing System VP 470 (US Patent #6,613,284 and Republic of China Invention Patent # NI-164,388, and European patent #1,363,737), this dual slide system places 768 spots ~500 microns in diameter onto a grid 18 mm wide and 36 mm tall on two separate slides. The ability to index two slides at the same time facilitates your ability to make duplicate slides. This also saves the time and effort required to wash the pins between slides. The pitch or center-to-center spacing between the spots is 750 microns on the Y axis and 1,125 microns in the X axis. This array will accommodate eight, 96 well microplates or two, 384 well microplates. However, you can use as few spots as your library requires or as spot size dictates. Our Replicator pins [FP1S6] have a 6 nl slot and liquid is transferred in that slot as well as in a hanging drop at the tip of the pin and on the sides of the pin [up to ~30 nl]. The diameter of the floating pins is 457 microns The pins must be reloaded after each spotting. Reloading the pins is as simple as dipping them in a microplate. The amount that is deposited on the slide will depend on the surface tension of the liquid and the surface tension of the slide. Usually between 3 and 13 nl is on a slide. The volume deposited on a membrane will be larger due to the capillary action of the membrane. The spot produced on silanated slides is usually ~ 500 microns in diameter. This spot size allows a 24 by 32 array or 768 spots/array; while on epoxy-coated slides, the spot is ~780 microns in diameter, so the highest density is 384 spots/epoxy-coated slide.
| Position | Mean | SD | %CV | Max | Min |
|---|---|---|---|---|---|
| Pin 1 | 793.1 | 42.7 | 0.054 | 920 | 700 |
| Pin 2 | 786.7 | 42.1 | 0.054 | 910 | 700 |
| Pin 3 | 789.2 | 40.5 | 0.051 | 890 | 710 |
| Pin 4 | 786.3 | 35.4 | 0.045 | 870 | 730 |
| Pin 5 | 787.5 | 39.1 | 0.050 | 910 | 710 |
| Pin 6 | 757.9 | 41.4 | 0.055 | 870 | 690 |
| Pin 7 | 795.8 | 41.7 | 0.052 | 930 | 730 |
| Pin 8 | 745.2 | 37.4 | 0.050 | 840 | 690 |
| TOTAL | 780.2 | 43.3 | 0.055 | 930 | 690 |
