Pin Tool Data | V & P Scientific, Inc.

Solid Pin Data

Delivery Data for Aqueous Solutions in 96 Format With Uncoated And /ah Hydrophobic Coated Pins

Transfer Of Horseradish Peroxidase In Tris Buffered Saline With Pin Tools

Pin	Description		nl Transferred	CV%
0.229 mm diameter (FP9)	Total Pin	Uncoated	7.41	2.4
		Hydrophobic	7.46	5.4
0.229 mm diameter (FP9)	Hanging Drop	Uncoated	N/A	N/A
		Hydrophobic	2.09	3.8
0.457 mm diameter (FP1)	Total Pin	Uncoated	33.48	3.2
		Hydrophobic	28.17	7.5
0.457 mm diameter (FP1)	Hanging Drop	Uncoated	16.96	4.5
		Hydrophobic	8.51	0.8
0.787 mm diameter (FP3)	Total Pin	Uncoated	87.32	3.9
		Hydrophobic	77.4	3.9
0.787 mm diameter (FP3)	Hanging Drop	Uncoated	48.77	1.2
		Hydrophobic	43.05	9.4
1.19 mm diameter (VP 409 & VP 386)	Total Pin	Uncoated	247.22	2.8
		Hydrophobic	192.67	2.6
1.19 mm diameter (VP 409 & VP 386)	Hanging Drop	Uncoated	76.35	1.6
		Hydrophobic	108.4	2.8
1.58 mm diameter (VP 408 & VP 384)	Total Pin	Uncoated	273.5	4.6
		Hydrophobic	259.25	3.1
1.58 mm diameter (VP 408 & VP 384)	Hanging Drop	Uncoated	201.93	5
		Hydrophobic	170.04	7.5

Total Pin = All the liquid on sides of pin plus the hanging drop that is transferred to a liquid filled well.
Hanging Drop = Only the liquid in the hanging drop that is transferred to a membrane.

Conclusions

Hydrophobic coating pins will reduce the total amount of aqueous HRP liquid transferred and also reduce the amount of non-specific binding to the stainless-steel pins. If the substance you are transferring has high non-specific binding this will be an important factor in selecting your pins.

Pin diameter also has an effect on the degree of reduction of liquid transfer with hydrophobic coating as the smaller the diameter the less the reduction of transfer. This is most likely due to the curvature of the pin affecting the wetting contact angle

Slot Pin Data

Delivery Data for DMSO Solutions in 96 Format With Uncoated And /ah Hydrophobic Coated Slot Pins

Transfer of Fluorescein 5-Isothiocyanate-Isomer I (FITC) In 100% DMSO To 1 M Tris, pH 8.0

Pin	Description			nl Transferred	CV%
0.457 mm diameter (FP1)	6 nl Slot	Total Pin*	Uncoated	25.6	10.8
			Hydrophobic	N/A	N/A
	10 nl Slot	Total Pin*	Uncoated	23.36	6.1
			Hydrophobic	25.85	6.9
	50 nl Slot	Total Pin*	Uncoated	67.83	2.5
			Hydrophobic	N/A	N/A
0.787 mm diameter (FP3)	100 nl Slot	Total Pin*	Uncoated	180.32	7.2
			Hydrophobic	205.84	5.5
	200 nl Slot	Total Pin*	Uncoated	277.82	4.9
			Hydrophobic	287.3	3.8
	500 nl Slot	Total Pin*	Uncoated	581.16	5.2
			Hydrophobic	555.69	3

Total Pin* = Volume of DMSO in Hanging Drop and on sides of the pin.

Conclusion

Hydrophobic coating pins will slightly increase the total amount of DMSO FITC liquid transferred.

Delivery Data for Aqueous Solutions in 96 Format With Uncoated And /ah Hydrophobic Coated Slot Pins

Transfer Of Horseradish Peroxidase In Tris Buffered Saline With Slot Pin Tools

Pin	Description		nl Transferred	CV%
0.787 mm diameter (FP3)	100 nl Slot Total Pin, Including Slot	Uncoated	195.69	1.6
		Hydrophobic	170.2	2.9
0.787 mm diameter (FP3)	100 nl Slot, Slot Only	Uncoated	149.67	4.9
		Hydrophobic	129.61	7.6
0.787 mm diameter (FP3)	200 nl Slot Total Pin, Including Slot	Uncoated	269.77	1.9
		Hydrophobic	228.62	17.1
0.787 mm diameter (FP3)	200 nl Slot, Slot Only	Uncoated	237.52	8.9
		Hydrophobic	186.9	5.9

Total Pin = All the liquid on the sides of the pins plus the slot that is transferred to a liquid filled well.
Slot Only = Only the liquid in the slot that is transferred to a membrane.

Conclusions

Although the slots in the pin are a precise volume, the liquid that is transferred is usually more because of the volume carried on the sides of the pins.

As seen with other aqueous data the amount transferred on hydrophobic coated Slot pins is less than on uncoated Solid or Slot pins. Thus Hydrophobic coating has the most effect on aqueous transfers.

Decreasing Source Plate Volume

Because the pins carry liquid on the sides as well as the tips and slots, the volume transferred by the pins will be affected by the depth the pin is submerged into the source plate. The following experiment addresses this phenomenon. The results illustrate the importance of having the same volume in all the source wells when using solid pins. This differs from slot pin transfers that are not significantly affected by source well volume. In that case, the larger the slot the less effect source plate volume has on the transfer volume. Additionally, Aqueous Transfers are slightly larger than DMSO transfers on uncoated pins than on Hydrophobic coated pins as the source well volume is decreased

Assay conditions:

Hydrophobic coated and uncoated, solid, and slot pins were dipped into varied volumes of FITC in 100% DMSO or 0.1M Tris Buffer in 96 well source plates (25, 50, 100, and 200 ul). Pins were removed from the source plate at a speed of 0.78 cm/sec. Pins then transferred the FITC to 200 ul 0.1 M Tris, pH 8.0 in a black polystyrene assay plate. The fluorescence was measured in a Victor Plate Reader from which the volume of FITC transferred was calculated. In the following table, the volume transferred from the 200 ul source plate was assigned the value of 100%, and the volumes transferred by the same pins from source plates with 100, 50, and 25 ul volumes were calculated as a percentage of this volume.

DMSO Transfers Rates on Uncoated Pins

Pin	200 ul	100 ul	50 ul	25 ul
	Source Plate Volume
FP9	100	70.9	49.4	28.1
FP8	100	64.9	41.6	N/A
FP1	100	71.3	53.5	37
FP1S6	100	80.3	66.8	N/A
FP1S10	100	83.5	71.2	N/A
FP1S20	100	86.9	77.2	N/A
FP1S50	100	89.3	83.4	N/A
FP3	100	82.5	66.9	63.9
FP3S100	100	93.1	83.8	84.6
FP3S200	100	95.8	89.6	91
FP6	100	81.7	68.9	78.7
FP3S500	100	98.5	93	94.9
FPS.5	100	90.4	77.1	81.4
FP6S.5	100	90.3	78.6	86.5
FPS	100	94	90.4	85.5
FP6S	100	94.6	89.8	83
FPS2	100	96.1	93.5	90.3
FP6S2	100	96.5	94.2	85.4
FPS5	100	97	95.3	87.5
FP6S5	100	93.1	94.8	89.8
FP4	100	61.4	49.8	24.3
FP	100	69.9	50.6	50.3

Aqueous Transfer Rates on Uncoated Pins

Pin	200 ul	100 ul	50 ul	25 ul
	Source Plate Volume
FP9	100	79.4	52.4	36
FP8	100	67.9	50.8	43.4
FP1	100	77.2	68.1	61
FP1S6	100	79	66.6	N/A
FP1S10	100	81.9	71.9	N/A
FP1S20	100	87.9	82.5	N/A
FP1S50	100	94.4	89.8	N/A
FP3	100	92	87.6	87.7
FP3S100	100	98.3	93.2	94.3
FP3S200	100	99.7	97.7	100.5
FP6	100	99.6	96.4	N/A
FP3S500	100	108.8	109.8	N/A
FPS.5	100	92.6	88	N/A
FP6S.5	100	100.1	96.2	N/A
FPS	100	99.7	95.3	N/A
FP6S	100	101.6	98.8	N/A
FPS2	100	99.7	96	N/A
FP6S2	100	100	98.5	N/A
FPS5	100	98.8	95.3	N/A
FP6S5	100	98.4	98.4	N/A
FP4	100	82.2	74.8	N/A
FP	100	63.8	47.4	N/A

DMSO Transfers Rates on Hydrophobic Pins

Pin	200 ul	100 ul	50 ul	25 ul
	Source Plate Volume
FP9	100	66.2	47.2	28.8
FP1	100	70.7	54.1	37.7
FP1S6	100	78.3	64.4	N/A
FP1S10	100	81	69.1	N/A
FP1S20	100	85.4	76.1	N/A
FP1S30	100	87.5	78.6	N/A
FP1S40	100	87.6	79.3	N/A
FP1S50	100	89.8	82.6	N/A
FP3	100	89.2	75.8	73.3
FP3S100	100	93.1	84.6	84.1
FP3S200	100	95.3	88.8	91.1
FP3S500	100	100.3	93.7	95.6

Aqueous Transfer Rates on Hydrophobic Pins

Pin	200 ul	100 ul	50 ul	25 ul
	Source Plate Volume
FP9	100	75.1	49.1	32.8
FP1	100	75.6	66.8	59.3
FP1S6	100	77.9	65.6	N/A
FP1S10	100	83.9	74	N/A
FP1S20	100	86.8	77.9	N/A
FP1S30	100	88	83	N/A
FP1S40	100	90.8	84.4	N/A
FP1S50	100	92.7	89.1	65.5
FP3	100	98.4	93	95
FP3S100	100	99.8	96	97.9
FP3S200	100	101.5	99.7	N/A
FP3S500	100	107.5	111.1	N/A

Liquid-To-Liquid Transfer

Effect of DNA or BSA concentration on Slot Pin transfers of Uncoated and Hydrophobic Coated Pins (FP3CS500)

Solvent/Sample	Concentration	CV%	nl FITC Transferred	CV%	nl FITC Transferred
		Uncoated	Uncoated	Hydrophobic Coated	Hydrophobic Coated
DMSO (-)	0	8.1	353.42	7.5	298.72
DMSO + DNA (mg/ml)	0.5	6.6	497.21	6.6	435.86
	0.25	9	432.49	4.1	391.93
	0.125	8.9	363.64	0.9	344.75
	0.0625	2.3	381.86	2	331.68
	0.0313	1.5	378.03	4.4	331.71
	0.0156	1.2	357.52	1.4	329.03
Tris (-)	0	4.9	577.31	7.2	493.53
Tris + DNA (mg/ml)	0.5	4.5	540.53	1.1	477.5
	0.25	4.6	518.21	6.1	456.75
	0.125	15.8	583.25	4.1	438.82
	0.0625	4.2	551.17	3.1	433.69
	0.0313	4.4	536.66	2.3	458.37
	0.0156	2.9	528.53	1.2	441.1
Tris + BSA (%)	4	5.4	462.13	11	409.27
	1	4	452.86	2.7	426.58
	0.25	11.7	456.45	1.3	408.72
	0.0625	1.1	445.22	6.5	393.07
	0.0156	3.7	462.85	3.9	430.2
	0.0039	1.5	493.54	2.2	437.29
	0.001	2.9	504.25	0.7	475.96

Conclusions

1. Increasing the concentration of DNA (sheared salmon sperm) to .25 mg/ml significantly increases the volume of DMSO liquid transferred for both coated and uncoated FP3S500 Slot Pins.
2. Increasing the concentration of DNA does not significantly increase the volume of Tris buffer (aqueous) transferred by both coated and uncoated FP3S500 Slot Pins.
3.Increasing the concentration of BSA (Bovine Serum Albumin) significantly decreases the volume of Tris buffer transferred by both coated and uncoated FP3S500 Slot Pins.
4. Hydrophobic coated FP3S500 Slot Pins transferred less DMSO – DNA and less Tris DNA and less Tris BSA than the uncoated FP3S500 Slot Pins.
5. Both coated and uncoated FP3S500 pins transfer significantly more aqueous solution than DMSO.

Effect Of DNA Or BSA Concentration On Slot Pin Transfers Of Uncoated And Hydrophobic Coated Pins (FP1CS50)

Solvent/Sample	Concentration	CV%	nl FITC Transferred	CV%	nl FITC Transferred
		Uncoated	Uncoated	Hydrophobic Coated	Hydrophobic Coated
DMSO (-)	0	4.2	49.38	2.1	49.31
DMSO + DNA (mg/ml)	0.5	4.9	51.24	2.6	56.79
	0.25	1.7	50.2	1.2	49.53
	0.125	1.5	51.27	2.3	49.77
	0.0625	2.2	49.34	4.1	48.19
	0.0313	1.2	49.03	0.2	50.23
	0.0156	2.4	45.9	1.4	46.64
Tris (-)	0	2.6	89.51	2.9	91.34
Tris + DNA (mg/ml)	0.5	7	77.11	0.6	84.62
	0.25	3.9	82.22	1.6	84.89
	0.125	3.9	85.42	1	85.08
	0.0625	1.5	85.36	2.8	85.03
	0.0313	2	84.52	3	88.19
	0.0156	2.6	82.92	2.8	83.2

Conclusions

1. In contrast to the FP3S500 data, increasing the concentration of DNA to .25 mg/ml does not significantly increase the volume of DMSO liquid transferred for both coated and uncoated FP1S50 Slot Pins.
2. Increasing the concentration of DNA does not significantly increase the volume of Tris buffer (aqueous) transferred by both coated and uncoated FP1S50 Slot Pins.
3. In contrast to the FP3S500 data, FP1S50 coated pins transferred about the same volume of DNA at all concentrations as did uncoated pins.
4. Both coated and uncoated FP1S50 pins transfer significantly more aqueous solution than DMSO.
5. The differences between the FP3S500 and the FP1S50 pin may be due to the different pin diameter’s effect on contact angle and therefore on the “wetting” of the pin. See diagrams on the link to / ah energy system.

Liquid-To-Dry Transfer

Effect of DNA or BSA concentration on Slot Pin touch-off transfers of uncoated and Hydrophobic Coated Pins on 96 well Polystyrene plates (FP3CS500)

Sample	Concentration	CV%	nl FITC Transferred	CV%	nl FITC Transferred
		Uncoated	Uncoated	Hydrophobic Coated	Hydrophobic Coated
DMSO (-)	0	42.8	9.39	20.5	7.43
DMSO + DNA (mg/ml)	0.5	16	5.47	24.2	5.81
	0.25	66.1	7.01	9.3	7.2
	0.125	15.4	5.25	11.8	8.23
	0.0625	56	8.3	31.1	6.73
	0.0313	32.2	6.91	10.4	8.13
	0.0156	10.6	7.93	30.2	5.64
Tris (-)	0	9.8	37.22	21.3	33.84
Tris + DNA (mg/ml)	0.5	4.9	38.17	25.8	31.59
	0.25	3.4	39.82	69.9	27.84
	0.125	8	40.35	17.2	32.34
	0.0625	6.8	36.72	13	33.16
	0.0313	3.6	38.31	8.6	34.93
	0.0156	14.6	35.3	14.5	34.96
Tris + BSA (%)	4	9.5	30.81	67.5	22.47
	1	4.9	32.09	4.2	29.87
	0.25	14.4	32.1	8.1	28.67
	0.0625	6	34.21	2.3	30.76
	0.0156	6.1	35.38	6.5	31.47
	0.0039	4.5	34.54	18.5	29.64
	0.001	9.9	40.94	15.5	34.7

Conclusions

1. Dry touch off transfers with DNA in DMSO are not very reproducible with either coated or uncoated FP3CS500 slot pins. This may be due to small percentage of liquid transferred to the dry plate.
2. Increasing the concentration of DNA did not significantly affect the volume of Tris buffer (aqueous) transferred by either coated or uncoated FP3S500 Slot Pins.
3.Increasing the concentration of BSA significantly decreases the volume of Tris buffer transferred by both coated and uncoated FP3S500 Slot Pins.
4. Hydrophobic coated FP3S500 Slot Pins transferred slightly less DMSO – DNA and less Tris DNA and less Tris BSA than the uncoated FP3S500 Slot Pins.
5. Both coated and uncoated FP3S500 pins transfer significantly more aqueous solution than DMSO.
6. Coated FP3S500 pins had higher CV’s than did uncoated pins.

Pin Type	Pin Diameter(mm)	Shape	Low Range(nL)²	High Range(nL)²
FP	1.58	Solid Pointed	175	594
FPS.5	1.58	500nL Slot	524	962
FPS	1.58	1000nL Slot	1056	1476
FPS2	1.58	2000nL Slot	1739	2174
FPS5	1.58	5000nL Slot	5150	4953
FP6	1.58	Solid Flat	465	960
FP6S.5	1.58	500nL Slot	934	1445
FP6S	1.58	1000nL Slot	1396	1930
FP6S2	1.58	2000nL Slot	2072	2637
FP6S5	1.58	5000nL Slot	4820	4693
Footnotes:(1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well for 96 Format and 75ul source plate volume per well for 384 Format

Pin Type	Pin Diameter (mm)	Shape	96 Format Low Range(nL)²	96 Format High Range(nL)²	384 Format Low Range(nL)³	384 Format High Range(nL)³
FP9H	0.229	Solid	4	10	3	8
FP8H	0.35	Solid	9	24	6	17
FP1H	0.457	Solid	15	39	9	27
FP1S6H	0.457	6nL Slot	23	49	14	32
FP1S10H	0.457	10nL Slot	29	53	20	38
FP1S20H	0.457	20nL Slot	35	59	26	43
FP1S30H	0.457	30nL Slot	47	69	35	53
FP1S40H	0.457	40nL Slot	54	75	41	58
FP1S50H	0.457	50nL Slot	69	90	57	73
FP3H	0.787	Solid	67	134	27	76
FP4H	0.91	Solid	95	189	32	102
FP3S100H	0.787	100nL Slot	170	227	108	164
FP3S200H	0.787	200nL Slot	266	320	190	239
FP3S500H	0.787	500nL Slot	520	542	416	456
FP4S1000H	0.91	1000nL Slot	932	1000	741	805
FP4S2000H	0.91	2000nL Slot	1571	1638	1351	1423
Footnotes: (1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well (3) 75ul source plate volume per well

Pin Type	Diameter (mm)	Shape	96 Format Low Range(nL)²	96 Format High Range(nL)²	384 Format Low Range(nL)³	384 Format High Range(nL)³
FP	1.58	Solid Pointed	147	411	168	395
FPS.5	1.58	500nL Slot	442	704	631	843
FPS	1.58	1000nL Slot	893	1130	1343	1498
FPS2	1.58	2000nL Slot	1911	2038	2607	2767
FPS5	1.58	5000nL Slot	3908	4296	5180	5253
FP6	1.58	Solid Flat	323	674	154	398
FP6S.5	1.58	500nL Slot	734	1042	855	1053
FP6S	1.58	1000nL Slot	1210	1500	1638	1717
FP6S2	1.58	2000nL Slot	2299	2384	2787	3068
FP6S5	1.58	5000nL Slot	4329	4656	5237	5245
Footnotes:(1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well (3) 75ul source plate volume per well

Solid Pin Data

Delivery Data for Aqueous Solutions in 96 Format With Uncoated And /ah Hydrophobic Coated Pins

Conclusions

Slot Pin Data

Delivery Data for DMSO Solutions in 96 Format With Uncoated And /ah Hydrophobic Coated Slot Pins

Conclusion

Delivery Data for Aqueous Solutions in 96 Format With Uncoated And /ah Hydrophobic Coated Slot Pins

Conclusions

Decreasing Source Plate Volume

Assay conditions:

DMSO Transfers Rates on Uncoated Pins

Aqueous Transfer Rates on Uncoated Pins

DMSO Transfers Rates on Hydrophobic Pins

Aqueous Transfer Rates on Hydrophobic Pins

Liquid-To-Liquid Transfer

Effect of DNA or BSA concentration on Slot Pin transfers of Uncoated and Hydrophobic Coated Pins (FP3CS500)

Conclusions

Effect Of DNA Or BSA Concentration On Slot Pin Transfers Of Uncoated And Hydrophobic Coated Pins (FP1CS50)

Conclusions

Liquid-To-Dry Transfer

Effect of DNA or BSA concentration on Slot Pin touch-off transfers of uncoated and Hydrophobic Coated Pins on 96 well Polystyrene plates (FP3CS500)

Conclusions

Company

Products

Hydrophobic Coated vs Non-Coated Pin Transfers

Solid Pin Delivery Data For Aqueous Solutions In 96 Format With Uncoated And /Ah Hydrophobic Coated Pins

Conclusion

Slot Pin Delivery Data For Aqueous Solutions In 96 Format With Uncoated And /Ah Hydrophobic Coated Pin

Conclusion

Liquid Surface Tension

Effect Of DNA Or BSA Concentration On Slot Pin Transfers Of Uncoated And Hydrophobic Coated Pins (FP3CS500)

Conclusions

Effect Of DNA Or BSA Concentration On Slot Pin Transfers Of Uncoated And Hydrophobic Coated Pins (FP1CS50)

Conclusions

Aqueous Transfer with Solid Pins

DMSO Transfer with Slot Pins

Aqueous Transfer with Slot Pins

Withdrawl Speeds Impact on Volume Transfer

Solid Pins More affected by Source Plate Volume

Volume Transferred For FP1 Pins (Uncoated) In 96 And 384 Formats

Volume Transferred For FP3 Pins (Uncoated) In 96 And 384 Formats

Aqueous Solutions Pin Transfer Volumes Ranges

Aqueous Solutions on Uncoated Pins in 96 Format Microplates(1)

Aqueous Solutions on Hydrophobic Pins in 96 Format Microplates(1)

Aqueous Solution on E-Clip, Uncoated Pins(1)

DMSO Pin Transfer Volume Range Charts

Uncoated Pins in 96 and 384 Format Microplates(1)

Hydrophobic-coated Pins in 96 and 384 Format Microplates(1)

E-Clip, Uncoated Pins, for 96 and 384 Format Microplates(1)