Application Heating Blocks

Applications

The applications for Heat Blocks in Chemistry and Biology especially when combined with mixing are almost too many to enumerate.

Our Tumble Stirrers and Heat Blocks are used in conjunction for a variety of applications such as accelerating chemical, enzymatic and biological reactions in:

Redox parallel synthesis to develop new drugs Endorsement
Photoredox synthesis to develop new drugs
Heating to different temperatures coupled with mixing to determine the solubility of a compound
Heating to different temperatures and mixing to determine the optimum temperature for the enzymatic hydrolysis of steryl glucosides
Heating and stirring chemical reactions to speed completion
Miniaturization of fermentations and chemical reactions
Drug Susceptibility Assays
Resoluabilize dried extracts from large screening libraries
Protein Expression Screen
Thorough mixing of heated slurries, viscous solutions, and emulsions
Thorough mixing of heated immiscible liquids
Resuspend chemical libraries after storage by heating and mixing
Dissolve solid compounds by heating and mixing
Bacterial Culture incubation and aeration by mixing for Adaptive Laboratory Evolution experiments
Heating and Aeration of microbial cultures to increase DNA or protein yield
Determination of Growth Rate, Metabolite Production, and Final Biomass in a Tumble Stirred and heated Culture Vessel

References

See articles referencing applications of our Heat Blocks.

Pin	Description		nl Transferred	CV%
0.229 mm diameter (FP9)	Total Pin	Uncoated	7.41	2.4
		Hydrophobic	7.46	5.4
0.229 mm diameter (FP9)	Hanging Drop	Uncoated	N/A	N/A
		Hydrophobic	2.09	3.8
0.457 mm diameter (FP1)	Total Pin	Uncoated	33.48	3.2
		Hydrophobic	28.17	7.5
0.457 mm diameter (FP1)	Hanging Drop	Uncoated	16.96	4.5
		Hydrophobic	8.51	0.8
0.787 mm diameter (FP3)	Total Pin	Uncoated	87.32	3.9
		Hydrophobic	77.4	3.9
0.787 mm diameter (FP3)	Hanging Drop	Uncoated	48.77	1.2
		Hydrophobic	43.05	9.4
1.19 mm diameter (VP 409 & VP 386)	Total Pin	Uncoated	247.22	2.8
		Hydrophobic	192.67	2.6
1.19 mm diameter (VP 409 & VP 386)	Hanging Drop	Uncoated	76.35	1.6
		Hydrophobic	108.4	2.8
1.58 mm diameter (VP 408 & VP 384)	Total Pin	Uncoated	273.5	4.6
		Hydrophobic	259.25	3.1
1.58 mm diameter (VP 408 & VP 384)	Hanging Drop	Uncoated	201.93	5
		Hydrophobic	170.04	7.5

Pin	Description		nl Transferred	CV%
0.229 mm diameter (FP9)	Total Pin	Uncoated	7.41	2.4
		Hydrophobic	7.46	5.4
0.229 mm diameter (FP9)	Hanging Drop	Uncoated	N/A	N/A
		Hydrophobic	2.09	3.8
0.457 mm diameter (FP1)	Total Pin	Uncoated	33.48	3.2
		Hydrophobic	28.17	7.5
0.457 mm diameter (FP1)	Hanging Drop	Uncoated	16.96	4.5
		Hydrophobic	8.51	0.8
0.787 mm diameter (FP3)	Total Pin	Uncoated	87.32	3.9
		Hydrophobic	77.4	3.9
0.787 mm diameter (FP3)	Hanging Drop	Uncoated	48.77	1.2
		Hydrophobic	43.05	9.4
1.19 mm diameter (VP 409 & VP 386)	Total Pin	Uncoated	247.22	2.8
		Hydrophobic	192.67	2.6
1.19 mm diameter (VP 409 & VP 386)	Hanging Drop	Uncoated	76.35	1.6
		Hydrophobic	108.4	2.8
1.58 mm diameter (VP 408 & VP 384)	Total Pin	Uncoated	273.5	4.6
		Hydrophobic	259.25	3.1
1.58 mm diameter (VP 408 & VP 384)	Hanging Drop	Uncoated	201.93	5
		Hydrophobic	170.04	7.5

Solvent/Sample	Concentration	CV%	nl FITC Transferred	CV%	nl FITC Transferred
		Uncoated	Uncoated	Hydrophobic Coated	Hydrophobic Coated
DMSO (-)	0	8.1	353.42	7.5	298.72
DMSO + DNA (mg/ml)	0.5	6.6	497.21	6.6	435.86
	0.25	9	432.49	4.1	391.93
	0.125	8.9	363.64	0.9	344.75
	0.0625	2.3	381.86	2	331.68
	0.0313	1.5	378.03	4.4	331.71
	0.0156	1.2	357.52	1.4	329.03
Tris (-)	0	4.9	577.31	7.2	493.53
Tris + DNA (mg/ml)	0.5	4.5	540.53	1.1	477.5
	0.25	4.6	518.21	6.1	456.75
	0.125	15.8	583.25	4.1	438.82
	0.0625	4.2	551.17	3.1	433.69
	0.0313	4.4	536.66	2.3	458.37
	0.0156	2.9	528.53	1.2	441.1
Tris + BSA (%)	4	5.4	462.13	11	409.27
	1	4	452.86	2.7	426.58
	0.25	11.7	456.45	1.3	408.72
	0.0625	1.1	445.22	6.5	393.07
	0.0156	3.7	462.85	3.9	430.2
	0.0039	1.5	493.54	2.2	437.29
	0.001	2.9	504.25	0.7	475.96

Solvent/Sample	Concentration	CV%	nl FITC Transferred	CV%	nl FITC Transferred
		Uncoated	Uncoated	Hydrophobic Coated	Hydrophobic Coated
DMSO (-)	0	4.2	49.38	2.1	49.31
DMSO + DNA (mg/ml)	0.5	4.9	51.24	2.6	56.79
	0.25	1.7	50.2	1.2	49.53
	0.125	1.5	51.27	2.3	49.77
	0.0625	2.2	49.34	4.1	48.19
	0.0313	1.2	49.03	0.2	50.23
	0.0156	2.4	45.9	1.4	46.64
Tris (-)	0	2.6	89.51	2.9	91.34
Tris + DNA (mg/ml)	0.5	7	77.11	0.6	84.62
	0.25	3.9	82.22	1.6	84.89
	0.125	3.9	85.42	1	85.08
	0.0625	1.5	85.36	2.8	85.03
	0.0313	2	84.52	3	88.19
	0.0156	2.6	82.92	2.8	83.2

Pin	Description		nl Transferred	CV%
0.229 mm diameter (FP9)	Total Pin	Uncoated	7.41	2.4
		Hydrophobic	7.46	5.4
0.229 mm diameter (FP9)	Hanging Drop	Uncoated	N/A	N/A
		Hydrophobic	2.09	3.8
0.457 mm diameter (FP1)	Total Pin	Uncoated	33.48	3.2
		Hydrophobic	28.17	7.5
0.457 mm diameter (FP1)	Hanging Drop	Uncoated	16.96	4.5
		Hydrophobic	8.51	0.8
0.787 mm diameter (FP3)	Total Pin	Uncoated	87.32	3.9
		Hydrophobic	77.4	3.9
0.787 mm diameter (FP3)	Hanging Drop	Uncoated	48.77	1.2
		Hydrophobic	43.05	9.4
1.19 mm diameter (VP 409 & VP 386)	Total Pin	Uncoated	247.22	2.8
		Hydrophobic	192.67	2.6
1.19 mm diameter (VP 409 & VP 386)	Hanging Drop	Uncoated	76.35	1.6
		Hydrophobic	108.4	2.8
1.58 mm diameter (VP 408 & VP 384)	Total Pin	Uncoated	273.5	4.6
		Hydrophobic	259.25	3.1
1.58 mm diameter (VP 408 & VP 384)	Hanging Drop	Uncoated	201.93	5
		Hydrophobic	170.04	7.5

Applications

References

Company

Products

Hydrophobic Coated vs Non-Coated Pin Transfers

Solid Pin Delivery Data For Aqueous Solutions In 96 Format With Uncoated And /Ah Hydrophobic Coated Pins

Conclusion

Slot Pin Delivery Data For Aqueous Solutions In 96 Format With Uncoated And /Ah Hydrophobic Coated Pin

Conclusion

Liquid Surface Tension

Effect Of DNA Or BSA Concentration On Slot Pin Transfers Of Uncoated And Hydrophobic Coated Pins (FP3CS500)

Conclusions

Effect Of DNA Or BSA Concentration On Slot Pin Transfers Of Uncoated And Hydrophobic Coated Pins (FP1CS50)

Conclusions

Aqueous Transfer with Solid Pins

DMSO Transfer with Slot Pins

Aqueous Transfer with Slot Pins

Withdrawl Speeds Impact on Volume Transfer

Solid Pins More affected by Source Plate Volume

Volume Transferred For FP1 Pins (Uncoated) In 96 And 384 Formats

Volume Transferred For FP3 Pins (Uncoated) In 96 And 384 Formats

Aqueous Solutions Pin Transfer Volumes Ranges

Aqueous Solutions on Uncoated Pins in 96 Format Microplates(1)

Aqueous Solutions on Hydrophobic Pins in 96 Format Microplates(1)

Aqueous Solution on E-Clip, Uncoated Pins(1)

DMSO Pin Transfer Volume Range Charts

Uncoated Pins in 96 and 384 Format Microplates(1)

Hydrophobic-coated Pins in 96 and 384 Format Microplates(1)

E-Clip, Uncoated Pins, for 96 and 384 Format Microplates(1)

Pin Type	Pin Diameter(mm)	Shape	96 Format Low Range(nL)²	96 Format High Range(nL)²
FP9	0.229	Solid	13	39
FP8	0.356	Solid	15	37
FP1	0.457	Solid	22	61
FP1S6	0.457	6nL Slot	34	67
FP1S10	0.457	10nL Slot	39	74
FP1S50	0.457	50nL Slot	90	124
FP3	0.787	Solid	93	213
FP3S100	0.787	100nL Slot	213	334
FP3S200	0.787	200nL Slot	311	449
FP3S500	0.787	500nL Slot	515	671
FP4	0.914	Solid	126	289
Footnotes: (1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well

Pin Type	Pin Diameter(mm)	Shape	96 Format Low Range(nL)²	96 Format High Range(nL)²
FP9	0.229	Solid	13	38
FP8	0.356	Solid
FP1	0.457	Solid	23	60
FP1S6	0.457	6nL Slot	33	67
FP1S10	0.457	10nL Slot	40	75
FP1S50	0.457	50nL Slot	86	119
FP3	0.787	Solid	76	209
FP3S100	0.787	100nL Slot	188	324
FP3S200	0.787	200nL Slot	288	436
FP3S500	0.787	500nL Slot	473	649
FP4	0.914	Solid
Footnotes: (1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well

Pin Type	Pin Diameter(mm)	Shape	Low Range(nL)²	High Range(nL)²
FP	1.58	Solid Pointed	175	594
FPS.5	1.58	500nL Slot	524	962
FPS	1.58	1000nL Slot	1056	1476
FPS2	1.58	2000nL Slot	1739	2174
FPS5	1.58	5000nL Slot	5150	4953
FP6	1.58	Solid Flat	465	960
FP6S.5	1.58	500nL Slot	934	1445
FP6S	1.58	1000nL Slot	1396	1930
FP6S2	1.58	2000nL Slot	2072	2637
FP6S5	1.58	5000nL Slot	4820	4693
Footnotes:(1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well for 96 Format and 75ul source plate volume per well for 384 Format

Pin Type	Pin Diameter(mm)	Shape	96 Format Low Range(nL)²	96 Format High Range(nL)²	384 Format Low Range(nL)³	384 Format High Range(nL)³
FP9	0.229	Solid	4	10	3	8
FP8	0.35	Solid	13	26	6	18
FP1	0.457	Solid	18	43	11	31
FP1S6	0.457	6nL Slot	24	49	15	34
FP1S10	0.457	10nL Slot	30	54	21	40
FP1S20	0.457	20nL Slot	37	61	27	46
FP1S30	0.457	30nL Slot	46	68	35	54
FP1S40	0.457	40nL Slot	57	78	45	63
FP1S50	0.457	50nL Slot	70	90	56	75
FP3	0.787	Solid	67	139	29	79
FP4	0.91	Solid	94	197	34	98
FP3S100	0.787	100nL Slot	175	241	114	163
FP3S200	0.787	200nL Slot	280	332	203	250
FP3S500	0.787	500nL Slot	535	559	427	464
FP4S1000	0.91	1000nL Slot	940	1011	704	800
FP4S2000	0.91	2000nL Slot	1518	1608	1277	1362
Footnotes: (1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well (3) 75ul source plate volume per well