Applications – Pin Tools

Robotic Pin Tool Applications Using 3nL to 5uL of Liquid

For:

• Drugs, Antibiotics, Receptors, Ligands, Activity on cultured cells, Mutants, Recombinant clones, Hybridomas,
• Nucleic acids, DNA, cDNA, RNA, mRNA, RNAi, PCR products
• Cells, Fungi, Yeast, Bacteria, Viruses, Plasmids

Application – Transfer and replicate cultures and genomic libraries

For:

• Cells, Fungi, Yeast, Bacteria, Viruses, Plasmids

Application – Assays on Membranes

For:

• Construction of high-density Nucleic Acid Arrays;
• Inexpensive Hand-held Device for the Construction of High-Density Nucleic Acid Arrays;
•  NIH Microarray Project Protocols

For:

• Gene expression profiles;
• Differential gene expression profiles between normal and cancerous tissues;
• RNA and DNA sequence analysis of gene expression;
• In Vivo Gene Expression Profile Analysis of Human Breast Cancer Progression;
• Differential Gene Expression Mapping and functional analysis of receptor-like protein kinases;
• Differential Gene Expression between Normal and Tumor-derived Ovarian Epithelial Cells;
• Changes in Gene Expression of Leaves in Sugarcane; Expressed Sequence Tags (EST) in cDNA;

For:

• Mapping and Functional Analysis of protein kinases;
• Development of DNA-Based Macroarray for the Detection and Identification of species;
• Physical Map of a  Chromosome in Nectria haematococca Mating Population VI;
• Development of a DNA-Based Macroarray for the Detection and Identification of Amanita Species;
• A High Through-put Procedure for Capturing Microsatellites from Complex Plant Genomes;

 For:

• Oligonucleotide Fingerprinting of Ribosomal RNA Genes;
• Oligonucleotide fingerprinting of rDNA genes;
• Characterization of fecal microbiota by oligonucleotide fingerprinting of rDNA genes;

Application – Assays on Glass Slide Arrays

For:

• Drug Resistance;
• Measurement of specific proteins in human cumulus cells using reverse phase protein array;
• Development of a universal peptide-binding protein for simultaneous assay of kinases;
• Specific proteins using reverse phase protein arrays; Micro-immunoassay Using a Protein Chip;

For:

• DNA – sequence analysis of gene expression;
• RNA – sequence analysis of gene expression;
• cDNA – sequence analysis of gene expression

Application – Assays on microplates for Multiplexing

For:

• DNA; RNA; Protein; Antibody; Antigen

Application – Assays on agar

For:

• Functional Genomic screens;
• Anti-microbial agents;
• Antibiotic resistance;
• Drug Resistance;
• Up-regulated proteins;
• Toxicity;
• Antisense RNA;
• Mutant strains;
• Receptors;
• High Throughput Reverse Genetic Screens;
• Genetic Architecture of Biofilm formation;

For:

• Genetic Determinants of swimming motility;
• Saccharomyces Gene Deletion project;
• Synthetic Genetic array  (SGA) analysis;
• Biologically active small molecules and the Yeast Halo Assay;
• Colony arraying with the VIRTEK CAPS system;
• Deletion of duplicate genes;

For:

• Discovery of Biologically Active Small Molecules Using a High-Throughput Yeast Halo Assay;
• A Halo-Based Potency Prediction Algorithm for High-Throughput Detection of Antimicrobial Agents;
• Functional Genomic Yeast Screen to Identify Pathogenic Bacterial Proteins;
• Genome-Wide Screen in Francisella novicida for Genes Required for Pulmonary and Systemic Infection;
• Harnessing Hsp90 function as a therapeutic strategy for fungal infectious disease

For:

• Detection of Different Cellular and Gene Expression Phenotypes;
• Drug Resistance of the Fungal Pathogen Candida albicans;
• Detecting TDP-43 toxicity in yeast;
• A genome-wide inducible phenotypic screen identifies antisense RNA constructs silencing Escherichia coli essential genes;
• High-Throughput Reverse Genetic Screening.

Application – Picking Colonies on Agar and Transferring to liquid culture

For:

• Fungi; Yeast; Bacteria;

Application – Yeast Mating Studies on agar

For:

•  Yeast genetics

Miscellaneous Applications

• Resuspend settled material or organisms;
• Reformat libraries (96-to-384, 384-to-1536, 1536-to-384, 384-to-96);
• Wounding Tissue Culture Monolayers with Pin Tools;
• Dysfunctional Connections Between the Nucleus and the Actin and Microtubule Networks in Laminopathic Models;
• A high-throughput cell migration assay using scratch wound healing, a comparison of image-based readout methods;
• A Multi-domain Fragment of Nogo-A Protein Is a Potent Inhibitor of Cortical Axon Regeneration via Nogo Receptor;