VP 550D

DOCKING STATION, for Disposable Pin Tools, accommodates both standard and deep well versions. The cutaway sides on the docking station allow for robotic grippers to come in from the top or side to pick up the disposable pin tool. By using the grippers to drop a disposable pin tool onto the docking station prior to performing the pin tool operation, the beveled edge will locate the disposable pin tool to the same position every time. This will ensure that the disposable pin tool is always picked up in the same location, which is very critical when working in a 384 format.

Plate Type	Deep Well
Mode of Use	For Robotic/Liquid Handler Use
Material	Polypropylene
SLAS Base	Yes

Disposable Pin Tool System Overview

We offer a Disposable Pin Tool System, either manual or robotic, that can transfer liquid from/to standard and deep well 96-well microplates and standard 384-well microplates. Each pin of the Disposable Pin Tools is either sterile or non-sterile (VP 246, VP 246A, VP 246A-NS, VP 246-NS, VP 248, or VP 248-NS) and delivers ~ 130nL.

The manual version of the Disposable Pin Tool System requires a Manual Adaptor (VP 240 or VP240A), a Loading Jig (VP 242 or VP 242A), a Library Copier™ (VP 244 or VP 244A), and the Disposable Pin Tool (VP 246, VP 246A, VP 246A-NS, VP 246-NS, VP 248 or VP 248-NS).

The robotic version of the Disposable Pin Tool System requires the Robotic Adapter (VP 240R) to be attached to a V&P Robotic Mounting Plate (see Robotic Pin Tools) specifically designed for the robot. The other needed parts include the Disposable Pin Tool Docking Station (VP 550D) and the Disposable Pin Tool Removal Station (VP 245R-1A).

For our customers with special requirements, our sterile disposable pin tools are a cost-effective solution for replicating genomic libraries and increasing throughput. Simply use the adaptor to load the sterile pin tool on the docking station and remove it. No washing, no blotting, just dip, dunk and dispose.

Pin	Description		nl Transferred	CV%
0.229 mm diameter (FP9)	Total Pin	Uncoated	7.41	2.4
		Hydrophobic	7.46	5.4
0.229 mm diameter (FP9)	Hanging Drop	Uncoated	N/A	N/A
		Hydrophobic	2.09	3.8
0.457 mm diameter (FP1)	Total Pin	Uncoated	33.48	3.2
		Hydrophobic	28.17	7.5
0.457 mm diameter (FP1)	Hanging Drop	Uncoated	16.96	4.5
		Hydrophobic	8.51	0.8
0.787 mm diameter (FP3)	Total Pin	Uncoated	87.32	3.9
		Hydrophobic	77.4	3.9
0.787 mm diameter (FP3)	Hanging Drop	Uncoated	48.77	1.2
		Hydrophobic	43.05	9.4
1.19 mm diameter (VP 409 & VP 386)	Total Pin	Uncoated	247.22	2.8
		Hydrophobic	192.67	2.6
1.19 mm diameter (VP 409 & VP 386)	Hanging Drop	Uncoated	76.35	1.6
		Hydrophobic	108.4	2.8
1.58 mm diameter (VP 408 & VP 384)	Total Pin	Uncoated	273.5	4.6
		Hydrophobic	259.25	3.1
1.58 mm diameter (VP 408 & VP 384)	Hanging Drop	Uncoated	201.93	5
		Hydrophobic	170.04	7.5

Pin	Description		nl Transferred	CV%
0.229 mm diameter (FP9)	Total Pin	Uncoated	7.41	2.4
		Hydrophobic	7.46	5.4
0.229 mm diameter (FP9)	Hanging Drop	Uncoated	N/A	N/A
		Hydrophobic	2.09	3.8
0.457 mm diameter (FP1)	Total Pin	Uncoated	33.48	3.2
		Hydrophobic	28.17	7.5
0.457 mm diameter (FP1)	Hanging Drop	Uncoated	16.96	4.5
		Hydrophobic	8.51	0.8
0.787 mm diameter (FP3)	Total Pin	Uncoated	87.32	3.9
		Hydrophobic	77.4	3.9
0.787 mm diameter (FP3)	Hanging Drop	Uncoated	48.77	1.2
		Hydrophobic	43.05	9.4
1.19 mm diameter (VP 409 & VP 386)	Total Pin	Uncoated	247.22	2.8
		Hydrophobic	192.67	2.6
1.19 mm diameter (VP 409 & VP 386)	Hanging Drop	Uncoated	76.35	1.6
		Hydrophobic	108.4	2.8
1.58 mm diameter (VP 408 & VP 384)	Total Pin	Uncoated	273.5	4.6
		Hydrophobic	259.25	3.1
1.58 mm diameter (VP 408 & VP 384)	Hanging Drop	Uncoated	201.93	5
		Hydrophobic	170.04	7.5

Solvent/Sample	Concentration	CV%	nl FITC Transferred	CV%	nl FITC Transferred
		Uncoated	Uncoated	Hydrophobic Coated	Hydrophobic Coated
DMSO (-)	0	8.1	353.42	7.5	298.72
DMSO + DNA (mg/ml)	0.5	6.6	497.21	6.6	435.86
	0.25	9	432.49	4.1	391.93
	0.125	8.9	363.64	0.9	344.75
	0.0625	2.3	381.86	2	331.68
	0.0313	1.5	378.03	4.4	331.71
	0.0156	1.2	357.52	1.4	329.03
Tris (-)	0	4.9	577.31	7.2	493.53
Tris + DNA (mg/ml)	0.5	4.5	540.53	1.1	477.5
	0.25	4.6	518.21	6.1	456.75
	0.125	15.8	583.25	4.1	438.82
	0.0625	4.2	551.17	3.1	433.69
	0.0313	4.4	536.66	2.3	458.37
	0.0156	2.9	528.53	1.2	441.1
Tris + BSA (%)	4	5.4	462.13	11	409.27
	1	4	452.86	2.7	426.58
	0.25	11.7	456.45	1.3	408.72
	0.0625	1.1	445.22	6.5	393.07
	0.0156	3.7	462.85	3.9	430.2
	0.0039	1.5	493.54	2.2	437.29
	0.001	2.9	504.25	0.7	475.96

Solvent/Sample	Concentration	CV%	nl FITC Transferred	CV%	nl FITC Transferred
		Uncoated	Uncoated	Hydrophobic Coated	Hydrophobic Coated
DMSO (-)	0	4.2	49.38	2.1	49.31
DMSO + DNA (mg/ml)	0.5	4.9	51.24	2.6	56.79
	0.25	1.7	50.2	1.2	49.53
	0.125	1.5	51.27	2.3	49.77
	0.0625	2.2	49.34	4.1	48.19
	0.0313	1.2	49.03	0.2	50.23
	0.0156	2.4	45.9	1.4	46.64
Tris (-)	0	2.6	89.51	2.9	91.34
Tris + DNA (mg/ml)	0.5	7	77.11	0.6	84.62
	0.25	3.9	82.22	1.6	84.89
	0.125	3.9	85.42	1	85.08
	0.0625	1.5	85.36	2.8	85.03
	0.0313	2	84.52	3	88.19
	0.0156	2.6	82.92	2.8	83.2

Pin	Description		nl Transferred	CV%
0.229 mm diameter (FP9)	Total Pin	Uncoated	7.41	2.4
		Hydrophobic	7.46	5.4
0.229 mm diameter (FP9)	Hanging Drop	Uncoated	N/A	N/A
		Hydrophobic	2.09	3.8
0.457 mm diameter (FP1)	Total Pin	Uncoated	33.48	3.2
		Hydrophobic	28.17	7.5
0.457 mm diameter (FP1)	Hanging Drop	Uncoated	16.96	4.5
		Hydrophobic	8.51	0.8
0.787 mm diameter (FP3)	Total Pin	Uncoated	87.32	3.9
		Hydrophobic	77.4	3.9
0.787 mm diameter (FP3)	Hanging Drop	Uncoated	48.77	1.2
		Hydrophobic	43.05	9.4
1.19 mm diameter (VP 409 & VP 386)	Total Pin	Uncoated	247.22	2.8
		Hydrophobic	192.67	2.6
1.19 mm diameter (VP 409 & VP 386)	Hanging Drop	Uncoated	76.35	1.6
		Hydrophobic	108.4	2.8
1.58 mm diameter (VP 408 & VP 384)	Total Pin	Uncoated	273.5	4.6
		Hydrophobic	259.25	3.1
1.58 mm diameter (VP 408 & VP 384)	Hanging Drop	Uncoated	201.93	5
		Hydrophobic	170.04	7.5

Disposable Pin Tool System Overview

Related Products

VP 240R

VP 245R-1A

VP 246

VP 246-NS

VP 246A

VP 248

VP 248-NS

Company

Products

Hydrophobic Coated vs Non-Coated Pin Transfers

Solid Pin Delivery Data For Aqueous Solutions In 96 Format With Uncoated And /Ah Hydrophobic Coated Pins

Conclusion

Slot Pin Delivery Data For Aqueous Solutions In 96 Format With Uncoated And /Ah Hydrophobic Coated Pin

Conclusion

Liquid Surface Tension

Effect Of DNA Or BSA Concentration On Slot Pin Transfers Of Uncoated And Hydrophobic Coated Pins (FP3CS500)

Conclusions

Effect Of DNA Or BSA Concentration On Slot Pin Transfers Of Uncoated And Hydrophobic Coated Pins (FP1CS50)

Conclusions

Aqueous Transfer with Solid Pins

DMSO Transfer with Slot Pins

Aqueous Transfer with Slot Pins

Withdrawl Speeds Impact on Volume Transfer

Solid Pins More affected by Source Plate Volume

Volume Transferred For FP1 Pins (Uncoated) In 96 And 384 Formats

Volume Transferred For FP3 Pins (Uncoated) In 96 And 384 Formats

Aqueous Solutions Pin Transfer Volumes Ranges

Aqueous Solutions on Uncoated Pins in 96 Format Microplates(1)

Aqueous Solutions on Hydrophobic Pins in 96 Format Microplates(1)

Aqueous Solution on E-Clip, Uncoated Pins(1)

DMSO Pin Transfer Volume Range Charts

Uncoated Pins in 96 and 384 Format Microplates(1)

Hydrophobic-coated Pins in 96 and 384 Format Microplates(1)

E-Clip, Uncoated Pins, for 96 and 384 Format Microplates(1)

Pin Type	Pin Diameter(mm)	Shape	96 Format Low Range(nL)²	96 Format High Range(nL)²
FP9	0.229	Solid	13	39
FP8	0.356	Solid	15	37
FP1	0.457	Solid	22	61
FP1S6	0.457	6nL Slot	34	67
FP1S10	0.457	10nL Slot	39	74
FP1S50	0.457	50nL Slot	90	124
FP3	0.787	Solid	93	213
FP3S100	0.787	100nL Slot	213	334
FP3S200	0.787	200nL Slot	311	449
FP3S500	0.787	500nL Slot	515	671
FP4	0.914	Solid	126	289
Footnotes: (1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well

Pin Type	Pin Diameter(mm)	Shape	96 Format Low Range(nL)²	96 Format High Range(nL)²
FP9	0.229	Solid	13	38
FP8	0.356	Solid
FP1	0.457	Solid	23	60
FP1S6	0.457	6nL Slot	33	67
FP1S10	0.457	10nL Slot	40	75
FP1S50	0.457	50nL Slot	86	119
FP3	0.787	Solid	76	209
FP3S100	0.787	100nL Slot	188	324
FP3S200	0.787	200nL Slot	288	436
FP3S500	0.787	500nL Slot	473	649
FP4	0.914	Solid
Footnotes: (1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well

Pin Type	Pin Diameter(mm)	Shape	Low Range(nL)²	High Range(nL)²
FP	1.58	Solid Pointed	175	594
FPS.5	1.58	500nL Slot	524	962
FPS	1.58	1000nL Slot	1056	1476
FPS2	1.58	2000nL Slot	1739	2174
FPS5	1.58	5000nL Slot	5150	4953
FP6	1.58	Solid Flat	465	960
FP6S.5	1.58	500nL Slot	934	1445
FP6S	1.58	1000nL Slot	1396	1930
FP6S2	1.58	2000nL Slot	2072	2637
FP6S5	1.58	5000nL Slot	4820	4693
Footnotes:(1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well for 96 Format and 75ul source plate volume per well for 384 Format

Pin Type	Pin Diameter(mm)	Shape	96 Format Low Range(nL)²	96 Format High Range(nL)²	384 Format Low Range(nL)³	384 Format High Range(nL)³
FP9	0.229	Solid	4	10	3	8
FP8	0.35	Solid	13	26	6	18
FP1	0.457	Solid	18	43	11	31
FP1S6	0.457	6nL Slot	24	49	15	34
FP1S10	0.457	10nL Slot	30	54	21	40
FP1S20	0.457	20nL Slot	37	61	27	46
FP1S30	0.457	30nL Slot	46	68	35	54
FP1S40	0.457	40nL Slot	57	78	45	63
FP1S50	0.457	50nL Slot	70	90	56	75
FP3	0.787	Solid	67	139	29	79
FP4	0.91	Solid	94	197	34	98
FP3S100	0.787	100nL Slot	175	241	114	163
FP3S200	0.787	200nL Slot	280	332	203	250
FP3S500	0.787	500nL Slot	535	559	427	464
FP4S1000	0.91	1000nL Slot	940	1011	704	800
FP4S2000	0.91	2000nL Slot	1518	1608	1277	1362
Footnotes: (1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well (3) 75ul source plate volume per well