VP 742D

VP 742D

HEAT BLOCK, 2 Deep Chambers, 120 Volts

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SKU VP 742D Categories , Tags ,

HEAT BLOCK, 2 Deep Chambers, Microplates or Racks of Tubes/Vials, Eddy Current Design to Heat & Stir, PID Controller, Temp 25 to 200 C, Mica insulation on Bottom to fit VP 710L5-1 Deck, 120 Volts

Chamber Depth

Deep

No of Chambers

2

Application

Heat and Stir

Temp Range

25 to 200 C

Power Supply Input

120 Volts

SLAS Base

Yes

Heat Block Overview

V&P Scientific builds robust, low-profile, ambient to 200oC Heat Blocks that enable heating and stirring in standard microplates, deep well microplates, or reaction blocks of glass tubes and sealed vials.  They are constructed out of a specially designed Eddy Current defeating aluminum designed to be used with our strong NdFeB Tumble Stirrers.  Although aluminum is not magnetic, when magnetic lines of flux pass through it, an eddy current of electricity is generated that heats the aluminum and causes the motor that is generating the magnetic field movement to work much harder.  That is why we invented the Eddy Current defeating design.  The low profile design also minimizes the distance from the samples to the magnetic field thus maximizing the stirring power of even viscous materials.

Each Heat Block comes with one or more SLAS Adaptor Bases made of Mica that are attached to the Heat Block’s underside. This base provides heat protection for surfaces below the Heat Block and facilitates its use in microplate-configured locations. The Mica is safety rated by UL laboratories as UL 94VO.  Another advantage of our design is that we have separated the control panel from the actual heating block. Thus, the Heat Block’s placement on the deck of a robotic workstation is seamless.

Our temperature controllers all use an RTD (Resistive Thermal Device) heat sensor.  RTD sensors are advantageous because they work in moving magnetic fields generated by V&P Tumble Stirrers while Thermocouples do not work in magnetic fields. This is important when heating and stirring simultaneously.

The VP 741 Series and VP 742 Series Heat Blocks have deep chambers and a lid that can be screwed into place to hold Cap Mats or other sealing materials in place while high temperatures are reached.  Heating and simultaneously stirring are very useful in redox chemical reactions.  Our deep chamber Heat Blocks all come with a U-shaped insert that allows easy removal of standard microplates or other short labware.

The VP 744 Series Heat Blocks are heated or cooled by recirculating hot or cold fluid in the heat exchange channels in the blocks.

The following graph contains data generated in a VP 741ABZ-R-MB Heat Block using a deep well 96 microplate with 2 ml of water in each well.   The RTD of the controller is embedded in the aluminum at the base of the Heat Block.  The Control temperature was set to 80° C and temperature readings were taken in microplate wells located at the middle (C6), side (A6), and corner (A12) positions and plotted against time.  As can be seen in the graph the temperature in the wells plateau at 4 to 6° below the set temperature of 80° C.   This difference between the set temperature and the well temperature needs to be determined and compensated for, in your system using your fluid, your volume, and your microplate or other vessels. This difference in set temperature and well temperature is due to the loss of heat transfer between the aluminum and the microplate and the fluid in the well.  Also note the best heat transfer occurs in the wells with the greatest contact with the aluminum of the Heat Block, and with time these approach the same temperature.  This is why we make Heat Block accessories that maximized the contact between the Heat Block and the microplate or vessel.

 

Applications

The applications for Heat Blocks in Chemistry and Biology especially when combined with mixing are almost too many to enumerate.

Our Tumble Stirrers and Heat Blocks are used in conjunction for a variety of applications such as accelerating chemical, enzymatic and biological reactions in:

  • Redox parallel synthesis to develop new drugs Endorsement
  • Photoredox synthesis to develop new drugs
  • Heating to different temperatures coupled with mixing to determine the solubility of a compound
  • Heating to different temperatures and mixing to determine the optimum temperature for the enzymatic hydrolysis of steryl glucosides
  • Heating and stirring chemical reactions to speed completion
  • Miniaturization of fermentations and chemical reactions
  • Drug Susceptibility Assays
  • Resoluabilize dried extracts from large screening libraries
  • Protein Expression Screen
  • Thorough mixing of heated  slurries, viscous solutions, and emulsions
  • Thorough mixing of heated  immiscible liquids
  • Resuspend chemical libraries after storage by heating and mixing
  • Dissolve solid compounds by heating and mixing
  • Bacterial Culture incubation and aeration by mixing for Adaptive Laboratory Evolution experiments
  • Heating and Aeration of microbial cultures to increase DNA or protein yield
  • Determination of Growth Rate, Metabolite Production, and Final Biomass in a Tumble Stirred and heated Culture Vessel

References
    See articles referencing applications of our Heat Blocks.

387 VP 742 Series Heating Blocks
380A_1 VP 741D Series Heating Blocks INKBIRD Instructions

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Hydrophobic Coated vs Non-Coated Pin Transfers

Solid Pin Delivery Data For Aqueous Solutions In 96 Format With Uncoated And /Ah Hydrophobic Coated Pins
PinDescriptionnl TransferredCV%
0.229 mm diameter (FP9)Total PinUncoated7.412.4
Hydrophobic7.465.4
0.229 mm diameter (FP9)Hanging DropUncoatedN/AN/A
Hydrophobic2.093.8
0.457 mm diameter (FP1)Total PinUncoated33.483.2
Hydrophobic28.177.5
0.457 mm diameter (FP1)Hanging DropUncoated16.964.5
Hydrophobic8.510.8
0.787 mm diameter (FP3)Total PinUncoated87.323.9
Hydrophobic77.43.9
0.787 mm diameter (FP3)Hanging DropUncoated48.771.2
Hydrophobic43.059.4
1.19 mm diameter  (VP 409 & VP 386)Total PinUncoated247.222.8
Hydrophobic192.672.6
1.19 mm diameter (VP 409 & VP 386)Hanging DropUncoated76.351.6
Hydrophobic108.42.8
1.58 mm diameter (VP 408 & VP 384)Total PinUncoated273.54.6
Hydrophobic259.253.1
1.58 mm diameter (VP 408 & VP 384)Hanging DropUncoated201.935
Hydrophobic170.047.5

Transfer Of Horseradish Peroxidase In Tris Buffered Saline With Pin Tools

Conclusion

Coating pins will reduce the total amount of liquid transferred and also reduce the amount of non-specific binding to the stainless-steel pins. If the substance you are transferring has high non-specific binding this will be an important factor in selecting your pins.

Slot Pin Delivery Data For Aqueous Solutions In 96 Format With Uncoated And /Ah Hydrophobic Coated Pin
PinDescriptionnl TransferredCV%
0.229 mm diameter (FP9)Total PinUncoated7.412.4
Hydrophobic7.465.4
0.229 mm diameter (FP9)Hanging DropUncoatedN/AN/A
Hydrophobic2.093.8
0.457 mm diameter (FP1)Total PinUncoated33.483.2
Hydrophobic28.177.5
0.457 mm diameter (FP1)Hanging DropUncoated16.964.5
Hydrophobic8.510.8
0.787 mm diameter (FP3)Total PinUncoated87.323.9
Hydrophobic77.43.9
0.787 mm diameter (FP3)Hanging DropUncoated48.771.2
Hydrophobic43.059.4
1.19 mm diameter  (VP 409 & VP 386)Total PinUncoated247.222.8
Hydrophobic192.672.6
1.19 mm diameter (VP 409 & VP 386)Hanging DropUncoated76.351.6
Hydrophobic108.42.8
1.58 mm diameter (VP 408 & VP 384)Total PinUncoated273.54.6
Hydrophobic259.253.1
1.58 mm diameter (VP 408 & VP 384)Hanging DropUncoated201.935
Hydrophobic170.047.5

Transfer Of Horseradish Peroxidase In Tris Buffered Saline With Pin Tools

Conclusion

Although the slots in the pin are a precise volume, the liquid that is transferred is usually more. The reason for this is due to the surface tension of the liquid causing the liquid in the slot to “bow out” thus increasing the volume of the liquid in the slot. If is important for you to transfer exactly a certain volume we can make custom slots to match the surface tension characteristics of your liquid

Liquid Surface Tension

Effect Of DNA Or BSA Concentration On Slot Pin Transfers Of Uncoated And Hydrophobic Coated Pins (FP3CS500)
Solvent/SampleConcentrationCV%nl FITC TransferredCV%nl FITC Transferred
UncoatedUncoatedHydrophobic CoatedHydrophobic Coated
DMSO (-)08.1353.427.5298.72
DMSO + DNA (mg/ml)0.56.6497.216.6435.86
0.259432.494.1391.93
0.1258.9363.640.9344.75
0.06252.3381.862331.68
0.03131.5378.034.4331.71
0.01561.2357.521.4329.03
Tris (-)04.9577.317.2493.53
Tris + DNA (mg/ml)0.54.5540.531.1477.5
0.254.6518.216.1456.75
0.12515.8583.254.1438.82
0.06254.2551.173.1433.69
0.03134.4536.662.3458.37
0.01562.9528.531.2441.1
Tris + BSA (%)45.4462.1311409.27
14452.862.7426.58
0.2511.7456.451.3408.72
0.06251.1445.226.5393.07
0.01563.7462.853.9430.2
0.00391.5493.542.2437.29
0.0012.9504.250.7475.96
Conclusions

1. Increasing the concentration of DNA (sheared salmon sperm) to .25 mg/ml significantly increases the volume of DMSO liquid transferred for both coated and uncoated FP3S500 Slot Pins.
2. Increasing the concentration of DNA does not significantly increase the volume of Tris buffer (aqueous) transferred by both coated and uncoated FP3S500 Slot Pins.
3. Increasing the concentration of BSA (Bovine Serum Albumin) significantly decreases the volume of Tris buffer transferred by both coated and uncoated FP3S500 Slot Pins.
4. Hydrophobic coated FP3S500 Slot Pins transferred less DMSO – DNA and less Tris DNA and less Tris BSA than the uncoated FP3S500 Slot Pins.
5. Both coated and uncoated FP3S500 pins transfer significantly more aqueous solution than DMSO.

Effect Of DNA Or BSA Concentration On Slot Pin Transfers Of Uncoated And Hydrophobic Coated Pins (FP1CS50)
Solvent/SampleConcentrationCV%nl FITC TransferredCV%nl FITC Transferred
UncoatedUncoatedHydrophobic CoatedHydrophobic Coated
DMSO (-)04.249.382.149.31
DMSO + DNA (mg/ml)0.54.951.242.656.79
0.251.750.21.249.53
0.1251.551.272.349.77
0.06252.249.344.148.19
0.03131.249.030.250.23
0.01562.445.91.446.64
Tris (-)02.689.512.991.34
Tris + DNA (mg/ml)0.5777.110.684.62
0.253.982.221.684.89
0.1253.985.42185.08
0.06251.585.362.885.03
0.0313284.52388.19
0.01562.682.922.883.2
Conclusions

1. In contrast to the FP3S500 data, increasing the concentration of DNA to .25 mg/ml does not significantly increase the volume of DMSO liquid transferred for both coated and uncoated FP1S50 Slot Pins.
2. Increasing the concentration of DNA does not significantly increase the volume of Tris buffer (aqueous) transferred by both coated and uncoated FP1S50 Slot Pins.
3. In contrast to the FP3S500 data, FP1S50 coated pins transferred about the same volume of DNA at all concentrations as did uncoated pins.
4. Both coated and uncoated FP1S50 pins transfer significantly more aqueous solution than DMSO.
5. The differences between the FP3S500 and the FP1S50 pin may be due to the different pin diameter’s effect on contact angle and therefore on the “wetting” of the pin. See the diagram on the link to / ah energy system.

PinDescriptionnl TransferredCV%
0.229 mm diameter (FP9)Total PinUncoated7.412.4
Hydrophobic7.465.4
0.229 mm diameter (FP9)Hanging DropUncoatedN/AN/A
Hydrophobic2.093.8
0.457 mm diameter (FP1)Total PinUncoated33.483.2
Hydrophobic28.177.5
0.457 mm diameter (FP1)Hanging DropUncoated16.964.5
Hydrophobic8.510.8
0.787 mm diameter (FP3)Total PinUncoated87.323.9
Hydrophobic77.43.9
0.787 mm diameter (FP3)Hanging DropUncoated48.771.2
Hydrophobic43.059.4
1.19 mm diameter  (VP 409 & VP 386)Total PinUncoated247.222.8
Hydrophobic192.672.6
1.19 mm diameter (VP 409 & VP 386)Hanging DropUncoated76.351.6
Hydrophobic108.42.8
1.58 mm diameter (VP 408 & VP 384)Total PinUncoated273.54.6
Hydrophobic259.253.1
1.58 mm diameter (VP 408 & VP 384)Hanging DropUncoated201.935
Hydrophobic170.047.5

Aqueous Transfer with Solid Pins

Hydrophobic coating pins will reduce the total amount of aqueous HRP liquid transferred and also reduce the amount of non-specific binding to the stainless-steel pins. If the substance you are transferring has high non-specific binding this will be an important factor in selecting your pins.

 

Pin diameter also has an effect on the degree of reduction of liquid transfer with hydrophobic coating as the smaller the diameter the less the reduction of transfer. This is most likely due to the curvature of the pin affecting the wetting contact angle

PinDescriptionnl TransferredCV%
0.457 mm diameter (FP1)6 nl SlotTotal Pin*Uncoated25.610.8
HydrophobicN/AN/A
10 nl SlotTotal Pin*Uncoated23.366.1
Hydrophobic25.856.9
50 nl SlotTotal Pin*Uncoated67.832.5
HydrophobicN/AN/A
0.787 mm diameter (FP3)  100 nl SlotTotal Pin*Uncoated180.327.2
Hydrophobic205.845.5
200 nl SlotTotal Pin*Uncoated277.824.9
Hydrophobic287.33.8
500 nl SlotTotal Pin*Uncoated581.165.2
Hydrophobic555.693

DMSO Transfer with Slot Pins

Hydrophobic coating pins will slightly increase the total amount of DMSO FITC liquid transferred.

PinDescriptionnl TransferredCV%
0.787 mm diameter (FP3)    100 nl Slot Total Pin, Including SlotUncoated195.691.6
Hydrophobic170.22.9
0.787 mm diameter (FP3)  100 nl Slot, Slot OnlyUncoated149.674.9
Hydrophobic129.617.6
0.787 mm diameter (FP3)200 nl Slot Total Pin, Including SlotUncoated269.771.9
Hydrophobic228.6217.1
0.787 mm diameter (FP3)200 nl Slot, Slot OnlyUncoated237.528.9
Hydrophobic186.95.9

Aqueous Transfer with Slot Pins

Although the slots in the pin are a precise volume, the liquid that is transferred is usually more because of the volume carried on the sides of the pins. 

As seen with other aqueous data the amount transferred on hydrophobic coated Slot pins is less than on uncoated Solid or Slot pins. Thus Hydrophobic coating has the most effect on aqueous transfers.

Withdrawl Speeds Impact on Volume Transfer

Solid Pins More affected by Source Plate Volume

Volume Transferred For FP1 Pins (Uncoated) In 96 And 384 Formats
Volume Transferred For FP3 Pins (Uncoated) In 96 And 384 Formats

Note: Same volume (200ul for 96 Format and 74 ul for 384 Format) in recipient plates and same pin withdrawal speed for all pins. Changes to pin withdrawal speed or volume in the source plate can result in different volumes being transferred.

Transfer volumes should always be confirmed by customers for their assay conditions and automated system.

Aqueous Solutions Pin Transfer Volumes Ranges

Aqueous Solutions on Uncoated Pins in 96 Format Microplates(1)
Pin TypePin Diameter(mm)Shape96 Format Low Range(nL)²96 Format High Range(nL)²
FP90.229Solid1339
FP80.356Solid1537
FP10.457Solid2261
FP1S60.4576nL Slot3467
FP1S100.45710nL Slot3974
FP1S500.45750nL Slot90124
FP30.787Solid93213
FP3S1000.787100nL Slot213334
FP3S2000.787200nL Slot311449
FP3S5000.787500nL Slot515671
FP40.914Solid126289
Footnotes: (1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well
Aqueous Solutions on Hydrophobic Pins in 96 Format Microplates(1)
Pin TypePin Diameter(mm)Shape96 Format Low Range(nL)²96 Format High Range(nL)²
FP90.229Solid1338
FP80.356Solid
FP10.457Solid2360
FP1S60.4576nL Slot3367
FP1S100.45710nL Slot4075
FP1S500.45750nL Slot86119
FP30.787Solid76209
FP3S1000.787100nL Slot188324
FP3S2000.787200nL Slot288436
FP3S5000.787500nL Slot473649
FP40.914Solid
Footnotes: (1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well
Aqueous Solution on E-Clip, Uncoated Pins(1)
Pin TypePin Diameter(mm)ShapeLow Range(nL)²High Range(nL)²
FP1.58Solid Pointed175594
FPS.51.58500nL Slot524962
FPS1.581000nL Slot10561476
FPS21.582000nL Slot17392174
FPS51.585000nL Slot51504953
FP61.58Solid Flat465960
FP6S.51.58500nL Slot9341445
FP6S1.581000nL Slot13961930
FP6S21.582000nL Slot20722637
FP6S51.585000nL Slot48204693
Footnotes:(1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well for 96 Format and 75ul source plate volume per well for 384 Format

DMSO Pin Transfer Volume Range Charts

Uncoated Pins in 96 and 384 Format Microplates(1)
Pin TypePin Diameter(mm)Shape96 Format Low Range(nL)²96 Format High Range(nL)²384 Format Low Range(nL)³384 Format High Range(nL)³
FP90.229Solid41038
FP80.35Solid1326618
FP10.457Solid18431131
FP1S60.4576nL Slot24491534
FP1S100.45710nL Slot30542140
FP1S200.45720nL Slot37612746
FP1S300.45730nL Slot46683554
FP1S400.45740nL Slot57784563
FP1S500.45750nL Slot70905675
FP30.787Solid671392979
FP40.91Solid941973498
FP3S1000.787100nL Slot175241114163
FP3S2000.787200nL Slot280332203250
FP3S5000.787500nL Slot535559427464
FP4S10000.911000nL Slot9401011704800
FP4S20000.912000nL Slot1518160812771362
Footnotes: (1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well (3) 75ul source plate volume per well
Hydrophobic-coated Pins in 96 and 384 Format Microplates(1)
Pin TypePin Diameter (mm)Shape96 Format Low Range(nL)²96 Format High Range(nL)²384 Format Low Range(nL)³384 Format High Range(nL)³
FP9H0.229Solid41038
FP8H0.35Solid924617
FP1H0.457Solid1539927
FP1S6H0.4576nL Slot23491432
FP1S10H0.45710nL Slot29532038
FP1S20H0.45720nL Slot35592643
FP1S30H0.45730nL Slot47693553
FP1S40H0.45740nL Slot54754158
FP1S50H0.45750nL Slot69905773
FP3H0.787Solid671342776
FP4H0.91Solid9518932102
FP3S100H0.787100nL Slot170227108164
FP3S200H0.787200nL Slot266320190239
FP3S500H0.787500nL Slot520542416456
FP4S1000H0.911000nL Slot9321000741805
FP4S2000H0.912000nL Slot1571163813511423
Footnotes: (1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well (3) 75ul source plate volume per well
E-Clip, Uncoated Pins, for 96 and 384 Format Microplates(1)
Pin TypeDiameter (mm)Shape96 Format Low Range(nL)²96 Format High Range(nL)²384 Format Low Range(nL)³384 Format High Range(nL)³
FP1.58Solid Pointed147411168395
FPS.51.58500nL Slot442704631843
FPS1.581000nL Slot893113013431498
FPS21.582000nL Slot1911203826072767
FPS51.585000nL Slot3908429651805253
FP61.58Solid Flat323674154398
FP6S.51.58500nL Slot73410428551053
FP6S1.581000nL Slot1210150016381717
FP6S21.582000nL Slot2299238427873068
FP6S51.585000nL Slot4329465652375245
Footnotes:(1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well (3) 75ul source plate volume per well