VP 772F3

Magnetic Bead Separation Racks and Devices for Bottles, Tubes and Micro-tubes:

V&P has developed over 37 different Magnetic Bead Separation Racks and devices for bottles, tubes, and micro-tubes, of nearly every description and covering the following range of rack size and vessel size.

• Single tube or bottle devices
• 4 tube or bottle racks, 6 tube rack, 8 tube rack, 10 tube rack, 12 tube rack, 14 tube rack, 16 tube rack, and a 24 tube rack,
• Bottle volumes of 100 ml, 500 ml, 1,000 ml, 2,000 ml and 5,000ml
• Tube volumes of 5 ml, 10 ml, 15 ml, 20 ml, and 50 ml
• Micro-tube volumes of 0.2 ml, 0.5 ml, 1.5 ml and 2.0 ml

Match the Bottle, Tube or Micro-tube to the Magnetic Bead Separation Device:

It is important to match the right magnetic bead separation device with your particular bottle or tube.  There are many characteristics of bottles and tubes that will affect where and how tightly the magnetic beads are pelleted:

• The thickness of the bottle or tube wall will determine how tightly the pellet is held.
• The smoothness of the interior surface will determine if beads will get trapped in crevices.
• The geometry of the vessel.
• The diameter of the vessel will determine how strong the magnets need to be.
• The shape and angle of the tube bottom will affect the angle of placement of the magnet.
• Your protocol will determine where the pellet should be located.

All of the above features are very important, and we have dealt with all of them over the course of the last 10 years that is why we say “choose your bottle or tube wisely before you launch a new assay protocol”.  With our large selection of magnetic separation devices, we can help you make the best decision for your application.  We are happy to share our knowledge with you.

Magnetic Beads:

We also consider the magnetic beads or particles you are using:

• Size of the beads
• Magnetic Moment
• Concentration

We may be able to suggest a specific product for your application. If the speed of separation is important to you, larger magnetic bead particles with greater magnetic moment will separate faster rather than weaker smaller particles.

Magnetic Bead Separation Location:

Depending on your protocol, you may want the beads to be collected:

• On the side of the wells
• High up the well
• Low down the well
• Or both high and low in the same Magnetic Separation device depending on different processes, elution, or wash volumes
• Directly in the bottom center of the well
• On the bottom outside ring of the well
• The mass or volume of the beads to be pelleted also determines which device is best
• The volume of supernatant and the method of removal (decanting, needle aspiration, or pipetting) are also important considerations

Magnetic Field Orientation and Strength:

The magnetic field orientation of adjacent magnets has a force multiplying effect (Halbach effect) and results in the magnetic beads being more tightly held:

• We use Halbach arrays in several of our designs to improve pelleting
• The normal magnetic field strength of the magnet also affects how tightly the beads are held
• How close the magnet is placed to the magnetic beads also affects how tightly the beads are held

Types of Supernatant Separation – Aspiration or Removal:

More commonly, magnetic beads are separated by aspiration using pipet tips and then washed. By using pipet tips, it is important to keep the beads away from the pipet. This is why bead pellet location and how tightly it is magnetically held are so important.  Similar consideration also applies to needle aspiration used in automatic plate washers.

V&P Scientific was the first to develop the Flick and Blot system for removing supernatant solutions from magnetic bead pellets. These devices allow for the microplate to be fixed to the magnet plate. You can then pick up the entire device and “Flick” out the supernatant into a sink or other container. This is a fast, economical way of removing a non-toxic supernatant from your microplates while still retaining your magnetic beads in the microplate for more rounds of washing.

Recently V&P Scientific developed a different method to separate the supernatant from the magnetic beads by using a 96 magnetic pin replicator (VP 407AM-N1) that is submerged into the wells and will remove the beads from the microplate and leave the supernatant behind in the microplate. Using a PCR plate as a shield, you can use our handheld magnetic pin tool device to move the beads from one microplate to another. This will save you time as it only takes a few seconds.  It will also save you money as no expensive pipet tips are needed, all you need is an inexpensive PCR plate.

Advantages of V&P Scientific’s Magnetic Separation Devices

• The largest number of devices produced by any manufacturer
• The greatest variety of devices to fit all Microplates
• Greatest variety for pellet location
• Strongest Magnets available in many different sizes
• Versions made for both BioTek and Tecan Hydroflex microplate washing systems
• Offered with and without an adjustable microplate registration system for precise alignment
• Different colored separation blocks for easy visualization (white, red, green, or gold)
• Custom Magnetic Separation Devices are not a problem
• Flexibility is our specialty

Accessories

See our accessories for aspirating supernatants, adding wash solutions, and for keeping magnetic beads uniformly in suspension prior to adding to microplate wells.  Recently we have developed a new SpinVessel^TM method that keeps magnetic beads in uniform suspension for aliquoting into microplates.