VP 708C5-7A
VP-708C5-7A-(1)
VP-708C5-7A-(2)
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VP-708C5-7A-(1)
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VP 708C5-7A

STIRRER, Vortex Lateral Tumble, Low Profile, Stand With Three Shelves To Hold Microplates, and SLAS Racks On All Four Sides

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SKU VP 708C5-7A Categories , , , Tags , , ,

STIRRER, Vortex Lateral Tumble, Servo Motor, High Speed and High Torque, Low Profile, Stand With Three Shelves To Hold Microplates and SLAS Racks On All Four Sides, Manual Control, RPM Readout on Control Unit LCD, 120/220 Volts, 60/50 Hz, CE Compliant

Container Used

Microplates, Syringes, Tubes/Vials
Mode of Use

Benchtop, Robotic Pipettor
Control Type

Manual Control
Magnetic Cylinder Length

177.8mm
Length

393.70mm
Width

152.40mm
Height

63.50mm
Motor Coupling

Direct Drive
Motor Specs

Closed System Servo, Double Stack, NEMA 23
Power Supply Output

48VDC, 5A
Power Supply Input

120/220V
Frequency

50/60 Hz
CE Compliant

Yes
Material

ABS
Magnet Strength

48MGO
Max RPM

1500
Drive System

Tumble Stirrer
Lateral Vortex  Tumble Stirrers – A Different Orientation

We created the world’s first LATERAL VORTEX by simply standing a Vertical Tumble Stirrer on end using a VP 710C5-7A  MICROPLATE TUMBLE STIRRER (USA Patent #7,484,880, European Union Patent #1,736,235 and German Patent #60 2006 026 122.5). Instead of tumbling a stir element in the vertical plane like our Alligator Tumble Stirrer, the Lateral Vortex Tumble Stirrer spins the stir elements by tumbling them laterally against a side wall of the vessel. This spinning motion produces a vortex cone in the liquid and efficiently mixes the contents of the liquid. This vortex cone is similar to the vortex cones produced by conventional horizontal magnetic stirrers however unlike the conventional horizontal stirrers which require a unique drive magnetic field under each vessel, the Lateral Vortex Tumble Stirrer because of its lateral wall tumbling action only requires one drive magnetic field to stir thousands of vessels. The Lateral Vortex Tumble Stirrers work best with round micro wells, or round tubes, vials, and bottles. Basically, our customers can convert our VP 710 series Tumble Stirrers into Lateral Vortex Stirrers by standing them on end with our accessory stands (VP 710C5-7A) which hold the stirrer vertically.

The Lateral Vortex Tumble Stirrer causes stir elements of all sizes and shapes to tumble laterally and create a vortex cone inside each well. The Lateral Vortex Tumble Stirrer will stir all types of microplates (24, 48, 96, 384 and even 1536 wells). It will also stir V and U bottom microplates, PCR plates, micro-centrifuge tubes, test tubes, vials and bottles. The only Stir elements that are not compatible with the Lateral Vortex Tumble Stirrer are the Stainless Steel Discs as the magnetic field in these stir elements is oriented through the diameter of the disc. These discs will simply spin in the wells causing little stirring action.

Advantages of the System
  • Can stir multiple, vials, tubes, large vessels, and microplates simultaneously
  • Complete stirring of large numbers of samples
  • Uniformity of stirring of wells throughout the microplate
  • Can run multiple experiments in parallel
  • Get more reactions per gram of starting material
  • Will stir even viscous solutions (honey & glycerol)
  • No cross contamination – wells do not have to be sealed
  • Simple to operate
  • Customizable to robotic systems
  • Can stir continuously for years without maintenance
  • Computer control option is available
V&P Scientific’s Unique and Patented Vertical Tumble Stirring Systems Applications

 

Multiple Tube or Microplate Stirring
  • Stir and mix
    • Any solution
    • Any shape
Magnetic Field Strength versus Distance from the Alligator Magnetic Tumble Stirrer Deck
Makers of cardiac pacemakers have placed magnetic switches in them that can be activated when the pacemaker is in a 5-gauss magnetic field. The critical 5 gauss line varies from 5.5″ to 22.8″ above the deck or cylinder guard with our Alligator Tumble Stirrers. The graph indicates that the magnetic field falls off very fast with distance. The distance above the deck to achieve a 5-gauss reading is 10″ for the VP 710C5-7A and 16″ for the VP 710E5X.

However, as a safety factor, we recommend that people with pacemakers keep at least 36″ or 91 cm from the Alligator Vertical Tumble Stirrers and the Vortex Lateral Tumble Stirrer. V&P does not make any claims in regard to the validity of the 5 gauss threshold for deactivating implanted medical devices. The 5 gauss magnetic field is a guideline to avoid risks associated with magnets and implanted medical devices such as pacemakers. For more information, please consult your physician.  We try to position the magnet as close to the deck as possible given the constraints of the magnetic cylinders and deck design. Note the distance between the magnet surface and the surface of the deck varies for each magnetic tumble stirrer design and thus the gauss levels at the surface of the deck vary.

463 Tumble Stirrer Operation – Servo Motors

Related Products

Since its founding in 1979 by Dr. Patrick Cleveland and his wife Victoria, V&P’s goal has been to provide the best engineered and most innovative research equipment available.

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Hydrophobic Coated vs Non-Coated Pin Transfers

Solid Pin Delivery Data For Aqueous Solutions In 96 Format With Uncoated And /Ah Hydrophobic Coated Pins
PinDescriptionnl TransferredCV%
0.229 mm diameter (FP9)Total PinUncoated7.412.4
Hydrophobic7.465.4
0.229 mm diameter (FP9)Hanging DropUncoatedN/AN/A
Hydrophobic2.093.8
0.457 mm diameter (FP1)Total PinUncoated33.483.2
Hydrophobic28.177.5
0.457 mm diameter (FP1)Hanging DropUncoated16.964.5
Hydrophobic8.510.8
0.787 mm diameter (FP3)Total PinUncoated87.323.9
Hydrophobic77.43.9
0.787 mm diameter (FP3)Hanging DropUncoated48.771.2
Hydrophobic43.059.4
1.19 mm diameter  (VP 409 & VP 386)Total PinUncoated247.222.8
Hydrophobic192.672.6
1.19 mm diameter (VP 409 & VP 386)Hanging DropUncoated76.351.6
Hydrophobic108.42.8
1.58 mm diameter (VP 408 & VP 384)Total PinUncoated273.54.6
Hydrophobic259.253.1
1.58 mm diameter (VP 408 & VP 384)Hanging DropUncoated201.935
Hydrophobic170.047.5

Transfer Of Horseradish Peroxidase In Tris Buffered Saline With Pin Tools

Conclusion

Coating pins will reduce the total amount of liquid transferred and also reduce the amount of non-specific binding to the stainless-steel pins. If the substance you are transferring has high non-specific binding this will be an important factor in selecting your pins.

Slot Pin Delivery Data For Aqueous Solutions In 96 Format With Uncoated And /Ah Hydrophobic Coated Pin
PinDescriptionnl TransferredCV%
0.229 mm diameter (FP9)Total PinUncoated7.412.4
Hydrophobic7.465.4
0.229 mm diameter (FP9)Hanging DropUncoatedN/AN/A
Hydrophobic2.093.8
0.457 mm diameter (FP1)Total PinUncoated33.483.2
Hydrophobic28.177.5
0.457 mm diameter (FP1)Hanging DropUncoated16.964.5
Hydrophobic8.510.8
0.787 mm diameter (FP3)Total PinUncoated87.323.9
Hydrophobic77.43.9
0.787 mm diameter (FP3)Hanging DropUncoated48.771.2
Hydrophobic43.059.4
1.19 mm diameter  (VP 409 & VP 386)Total PinUncoated247.222.8
Hydrophobic192.672.6
1.19 mm diameter (VP 409 & VP 386)Hanging DropUncoated76.351.6
Hydrophobic108.42.8
1.58 mm diameter (VP 408 & VP 384)Total PinUncoated273.54.6
Hydrophobic259.253.1
1.58 mm diameter (VP 408 & VP 384)Hanging DropUncoated201.935
Hydrophobic170.047.5

Transfer Of Horseradish Peroxidase In Tris Buffered Saline With Pin Tools

Conclusion

Although the slots in the pin are a precise volume, the liquid that is transferred is usually more. The reason for this is due to the surface tension of the liquid causing the liquid in the slot to “bow out” thus increasing the volume of the liquid in the slot. If is important for you to transfer exactly a certain volume we can make custom slots to match the surface tension characteristics of your liquid

Liquid Surface Tension

Effect Of DNA Or BSA Concentration On Slot Pin Transfers Of Uncoated And Hydrophobic Coated Pins (FP3CS500)
Solvent/SampleConcentrationCV%nl FITC TransferredCV%nl FITC Transferred
UncoatedUncoatedHydrophobic CoatedHydrophobic Coated
DMSO (-)08.1353.427.5298.72
DMSO + DNA (mg/ml)0.56.6497.216.6435.86
0.259432.494.1391.93
0.1258.9363.640.9344.75
0.06252.3381.862331.68
0.03131.5378.034.4331.71
0.01561.2357.521.4329.03
Tris (-)04.9577.317.2493.53
Tris + DNA (mg/ml)0.54.5540.531.1477.5
0.254.6518.216.1456.75
0.12515.8583.254.1438.82
0.06254.2551.173.1433.69
0.03134.4536.662.3458.37
0.01562.9528.531.2441.1
Tris + BSA (%)45.4462.1311409.27
14452.862.7426.58
0.2511.7456.451.3408.72
0.06251.1445.226.5393.07
0.01563.7462.853.9430.2
0.00391.5493.542.2437.29
0.0012.9504.250.7475.96
Conclusions

1. Increasing the concentration of DNA (sheared salmon sperm) to .25 mg/ml significantly increases the volume of DMSO liquid transferred for both coated and uncoated FP3S500 Slot Pins.
2. Increasing the concentration of DNA does not significantly increase the volume of Tris buffer (aqueous) transferred by both coated and uncoated FP3S500 Slot Pins.
3. Increasing the concentration of BSA (Bovine Serum Albumin) significantly decreases the volume of Tris buffer transferred by both coated and uncoated FP3S500 Slot Pins.
4. Hydrophobic coated FP3S500 Slot Pins transferred less DMSO – DNA and less Tris DNA and less Tris BSA than the uncoated FP3S500 Slot Pins.
5. Both coated and uncoated FP3S500 pins transfer significantly more aqueous solution than DMSO.

Effect Of DNA Or BSA Concentration On Slot Pin Transfers Of Uncoated And Hydrophobic Coated Pins (FP1CS50)
Solvent/SampleConcentrationCV%nl FITC TransferredCV%nl FITC Transferred
UncoatedUncoatedHydrophobic CoatedHydrophobic Coated
DMSO (-)04.249.382.149.31
DMSO + DNA (mg/ml)0.54.951.242.656.79
0.251.750.21.249.53
0.1251.551.272.349.77
0.06252.249.344.148.19
0.03131.249.030.250.23
0.01562.445.91.446.64
Tris (-)02.689.512.991.34
Tris + DNA (mg/ml)0.5777.110.684.62
0.253.982.221.684.89
0.1253.985.42185.08
0.06251.585.362.885.03
0.0313284.52388.19
0.01562.682.922.883.2
Conclusions

1. In contrast to the FP3S500 data, increasing the concentration of DNA to .25 mg/ml does not significantly increase the volume of DMSO liquid transferred for both coated and uncoated FP1S50 Slot Pins.
2. Increasing the concentration of DNA does not significantly increase the volume of Tris buffer (aqueous) transferred by both coated and uncoated FP1S50 Slot Pins.
3. In contrast to the FP3S500 data, FP1S50 coated pins transferred about the same volume of DNA at all concentrations as did uncoated pins.
4. Both coated and uncoated FP1S50 pins transfer significantly more aqueous solution than DMSO.
5. The differences between the FP3S500 and the FP1S50 pin may be due to the different pin diameter’s effect on contact angle and therefore on the “wetting” of the pin. See the diagram on the link to / ah energy system.

PinDescriptionnl TransferredCV%
0.229 mm diameter (FP9)Total PinUncoated7.412.4
Hydrophobic7.465.4
0.229 mm diameter (FP9)Hanging DropUncoatedN/AN/A
Hydrophobic2.093.8
0.457 mm diameter (FP1)Total PinUncoated33.483.2
Hydrophobic28.177.5
0.457 mm diameter (FP1)Hanging DropUncoated16.964.5
Hydrophobic8.510.8
0.787 mm diameter (FP3)Total PinUncoated87.323.9
Hydrophobic77.43.9
0.787 mm diameter (FP3)Hanging DropUncoated48.771.2
Hydrophobic43.059.4
1.19 mm diameter  (VP 409 & VP 386)Total PinUncoated247.222.8
Hydrophobic192.672.6
1.19 mm diameter (VP 409 & VP 386)Hanging DropUncoated76.351.6
Hydrophobic108.42.8
1.58 mm diameter (VP 408 & VP 384)Total PinUncoated273.54.6
Hydrophobic259.253.1
1.58 mm diameter (VP 408 & VP 384)Hanging DropUncoated201.935
Hydrophobic170.047.5

Aqueous Transfer with Solid Pins

Hydrophobic coating pins will reduce the total amount of aqueous HRP liquid transferred and also reduce the amount of non-specific binding to the stainless-steel pins. If the substance you are transferring has high non-specific binding this will be an important factor in selecting your pins.

 

Pin diameter also has an effect on the degree of reduction of liquid transfer with hydrophobic coating as the smaller the diameter the less the reduction of transfer. This is most likely due to the curvature of the pin affecting the wetting contact angle

PinDescriptionnl TransferredCV%
0.457 mm diameter (FP1)6 nl SlotTotal Pin*Uncoated25.610.8
HydrophobicN/AN/A
10 nl SlotTotal Pin*Uncoated23.366.1
Hydrophobic25.856.9
50 nl SlotTotal Pin*Uncoated67.832.5
HydrophobicN/AN/A
0.787 mm diameter (FP3)  100 nl SlotTotal Pin*Uncoated180.327.2
Hydrophobic205.845.5
200 nl SlotTotal Pin*Uncoated277.824.9
Hydrophobic287.33.8
500 nl SlotTotal Pin*Uncoated581.165.2
Hydrophobic555.693

DMSO Transfer with Slot Pins

Hydrophobic coating pins will slightly increase the total amount of DMSO FITC liquid transferred.

PinDescriptionnl TransferredCV%
0.787 mm diameter (FP3)    100 nl Slot Total Pin, Including SlotUncoated195.691.6
Hydrophobic170.22.9
0.787 mm diameter (FP3)  100 nl Slot, Slot OnlyUncoated149.674.9
Hydrophobic129.617.6
0.787 mm diameter (FP3)200 nl Slot Total Pin, Including SlotUncoated269.771.9
Hydrophobic228.6217.1
0.787 mm diameter (FP3)200 nl Slot, Slot OnlyUncoated237.528.9
Hydrophobic186.95.9

Aqueous Transfer with Slot Pins

Although the slots in the pin are a precise volume, the liquid that is transferred is usually more because of the volume carried on the sides of the pins. 

As seen with other aqueous data the amount transferred on hydrophobic coated Slot pins is less than on uncoated Solid or Slot pins. Thus Hydrophobic coating has the most effect on aqueous transfers.

Withdrawl Speeds Impact on Volume Transfer

Solid Pins More affected by Source Plate Volume

Volume Transferred For FP1 Pins (Uncoated) In 96 And 384 Formats
Volume Transferred For FP3 Pins (Uncoated) In 96 And 384 Formats

Note: Same volume (200ul for 96 Format and 74 ul for 384 Format) in recipient plates and same pin withdrawal speed for all pins. Changes to pin withdrawal speed or volume in the source plate can result in different volumes being transferred.

Transfer volumes should always be confirmed by customers for their assay conditions and automated system.

Aqueous Solutions Pin Transfer Volumes Ranges

Aqueous Solutions on Uncoated Pins in 96 Format Microplates(1)
Pin TypePin Diameter(mm)Shape96 Format Low Range(nL)²96 Format High Range(nL)²
FP90.229Solid1339
FP80.356Solid1537
FP10.457Solid2261
FP1S60.4576nL Slot3467
FP1S100.45710nL Slot3974
FP1S500.45750nL Slot90124
FP30.787Solid93213
FP3S1000.787100nL Slot213334
FP3S2000.787200nL Slot311449
FP3S5000.787500nL Slot515671
FP40.914Solid126289
Footnotes: (1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well
Aqueous Solutions on Hydrophobic Pins in 96 Format Microplates(1)
Pin TypePin Diameter(mm)Shape96 Format Low Range(nL)²96 Format High Range(nL)²
FP90.229Solid1338
FP80.356Solid
FP10.457Solid2360
FP1S60.4576nL Slot3367
FP1S100.45710nL Slot4075
FP1S500.45750nL Slot86119
FP30.787Solid76209
FP3S1000.787100nL Slot188324
FP3S2000.787200nL Slot288436
FP3S5000.787500nL Slot473649
FP40.914Solid
Footnotes: (1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well
Aqueous Solution on E-Clip, Uncoated Pins(1)
Pin TypePin Diameter(mm)ShapeLow Range(nL)²High Range(nL)²
FP1.58Solid Pointed175594
FPS.51.58500nL Slot524962
FPS1.581000nL Slot10561476
FPS21.582000nL Slot17392174
FPS51.585000nL Slot51504953
FP61.58Solid Flat465960
FP6S.51.58500nL Slot9341445
FP6S1.581000nL Slot13961930
FP6S21.582000nL Slot20722637
FP6S51.585000nL Slot48204693
Footnotes:(1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well for 96 Format and 75ul source plate volume per well for 384 Format

DMSO Pin Transfer Volume Range Charts

Uncoated Pins in 96 and 384 Format Microplates(1)
Pin TypePin Diameter(mm)Shape96 Format Low Range(nL)²96 Format High Range(nL)²384 Format Low Range(nL)³384 Format High Range(nL)³
FP90.229Solid41038
FP80.35Solid1326618
FP10.457Solid18431131
FP1S60.4576nL Slot24491534
FP1S100.45710nL Slot30542140
FP1S200.45720nL Slot37612746
FP1S300.45730nL Slot46683554
FP1S400.45740nL Slot57784563
FP1S500.45750nL Slot70905675
FP30.787Solid671392979
FP40.91Solid941973498
FP3S1000.787100nL Slot175241114163
FP3S2000.787200nL Slot280332203250
FP3S5000.787500nL Slot535559427464
FP4S10000.911000nL Slot9401011704800
FP4S20000.912000nL Slot1518160812771362
Footnotes: (1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well (3) 75ul source plate volume per well
Hydrophobic-coated Pins in 96 and 384 Format Microplates(1)
Pin TypePin Diameter (mm)Shape96 Format Low Range(nL)²96 Format High Range(nL)²384 Format Low Range(nL)³384 Format High Range(nL)³
FP9H0.229Solid41038
FP8H0.35Solid924617
FP1H0.457Solid1539927
FP1S6H0.4576nL Slot23491432
FP1S10H0.45710nL Slot29532038
FP1S20H0.45720nL Slot35592643
FP1S30H0.45730nL Slot47693553
FP1S40H0.45740nL Slot54754158
FP1S50H0.45750nL Slot69905773
FP3H0.787Solid671342776
FP4H0.91Solid9518932102
FP3S100H0.787100nL Slot170227108164
FP3S200H0.787200nL Slot266320190239
FP3S500H0.787500nL Slot520542416456
FP4S1000H0.911000nL Slot9321000741805
FP4S2000H0.912000nL Slot1571163813511423
Footnotes: (1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well (3) 75ul source plate volume per well
E-Clip, Uncoated Pins, for 96 and 384 Format Microplates(1)
Pin TypeDiameter (mm)Shape96 Format Low Range(nL)²96 Format High Range(nL)²384 Format Low Range(nL)³384 Format High Range(nL)³
FP1.58Solid Pointed147411168395
FPS.51.58500nL Slot442704631843
FPS1.581000nL Slot893113013431498
FPS21.582000nL Slot1911203826072767
FPS51.585000nL Slot3908429651805253
FP61.58Solid Flat323674154398
FP6S.51.58500nL Slot73410428551053
FP6S1.581000nL Slot1210150016381717
FP6S21.582000nL Slot2299238427873068
FP6S51.585000nL Slot4329465652375245
Footnotes:(1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well (3) 75ul source plate volume per well