Stirrers

What Makes Our Stirrers Unique

V&P Scientific, Inc. uses the largest and most powerful magnets in our Tumble, Levitation and Horizontal Stirrers. We have developed and patented five unique methods of uniformly mixing the contents of each well of a microplate or rack of tubes regardless of the position of the well or tube within the microplate or rack format. This is a feature that is not possible with conventional magnetic mixers.

5 Different
Methods

V&P holds five unique stirring + suspending patents.

Large & Strong
Magnets

NdFeB Magnets stir inexpensive stainless steel stir elements, facilitating throughput and economy.

Mix Highly
Viscous Material

Our Stirrers and NdFeB stir elements mix solutions 6.6 times more viscous than honey.

Increase Speed of
Chemical Reactions

Our stirrers can heat and uniformly mix simultaneously to speed reactions.

Largest Range
of Vessels

Our Stirrers and stir elements can stir vessels from 600 gallons to 10 ul.

Automation
Friendly

Can be located under or on the deck of a Robotic Liquid handler.

The VP Stirring Series

vp710

VP 710 Series

Vertical Tumble Stirrer

The Vertical Tumble Stirrer uses patented Magnetic Stir-Cylinder technology to mix large numbers of samples in microplates, racks of tubes, syringes, and small bottles (USA Patent #6,176,609).

Instead of spinning a magnetic stir bar about the horizontal plane like the standard horizontal magnetic flask stirrer, the Vertical Tumble Stirrer causes stir elements of all sizes and shapes to tumble vertically end-over-end inside each well or vessel. So each microplate well or tube in a rack, syringe, or small bottle receives the same vigorous mixing regardless of the position within the microplate or rack.

The Alligator Vertical Tumble Stirrer will stir all types of microplates of any volume or format. It will also stir V and U bottom microplates, PCR plates, micro-centrifuge tubes, test tubes, syringes, and small bottles, and specially designed Bubble Paddle reservoirs. Perhaps the best way to appreciate how the Vertical Tumble Stirrers function is to see all the videos above and below.

Mix the contents of any Microplate, Tube, Rack, Small Bottle, or Syringe.

  • Process Parallel synthetic reactions
  • Facilitates Micro-fermentation reactions
  • Parallel Artificial Membrane system to evaluate absorption
  • Mix and aerate cultures to increase yield
  • Solubilize compounds dried in microplates
  • Mix bread dough
  • Quantitative measure of cytotoxicity of anticancer drugs and other agents
  • High throughput solubility determination with application to the selection of compounds for fragment screening
  • Parallel fed-batch cultivations in micro bioreactors
  • High-throughput cocrystal slurry screening
  • Solubility studies
  • Adaptive Laboratory Evolution
  • Growth Rate, Metabolite Production, and Final Biomass in a Tumble Stirred Culture Vessel
  • Measurement of oxygen consumption by murine tissues in vitro
  • Miniaturization of fermentations and chemical reactions
  • Stirring chemical reactions to speed completion
  • Simultaneous stirring at 3 different temperatures to determine optimum temperature for a reaction
  • Heat and Stir to facilitate compound synthesis
  • Rapid and efficient stirring of any format microplates, test tubes, vials, and syringes
  • Thorough mixing of slurries, viscous solutions, and emulsions
  • Thorough mixing of immiscible liquids
  • Keep particulates in suspension
  • Resuspend chemical libraries after storage
  • Aeration of microbial cultures to increase DNA or protein yield
  • Enables parallel synthetic development of processes
  • Resuspend settled microorganisms
  • Keep microorganisms suspended during gridding operations
  • Break up filamentous organisms
  • Dissolve solid compounds
  • Resoluabilize dried extracts from large screening libraries
  • Mix and immobilize reactions that use magnetic beads
  1. Large and Strong Magnetic Cylinders
    • Allows use of Inexpensive Stir Elements
  2. High Throughput
    • Stir 11,520 Wells at Once
  3. Heat (200°C) AND Stir
    • Speeds Chemical Reactions
  4. Run Multiple Experiments in Parallel
  5. Flexible Orientation of Stir Deck and Heat Shields
    • Easily Customizable
    • Up to Three Temperature Zones
  6. Compatible with Most Robotic Liquid Handlers
  7. Stirs Bubble Paddle Reservoirs
  8. Set Exact RPM’s
    • Enables Reproducible Results Day-to-Day
  9. Durable
    • Stir Continuously for Years Without Maintenance
  10. Stir Viscous Solutions
    • 6.6 Times More Viscous than Honey
  1.  

Using large and the most powerful permanent magnets manufactured, we make many different models of Vertical Tumble Stirrers to fit the many varied applications and requirements of our customers. Requirements for heating and continuous stirring for a long period of time demand special Vertical Tumble Stirrers that are resistant to heat and with motors capable of working under high loads for extended periods.

We make our Vertical Tumble Stirrers heat resistant using Mica. The Mica deck will accommodate three of our V&P heat blocks operating at 200C. Please check with our knowledgeable staff to help you make the appropriate selection for your application. Our specialty is flexibility- let us know if you have a custom application today!

  1.  

We offer a large assortment of economical magnetic Stainless Steel Stir Elements from Stir StiXs to Discs to bars and dowels to fit into any well, tube, or syringe.

We also sell Alnico and Rare Earth Magnetic Stir Elements for more viscous solutions.

Check out the Stir Elements page for more information on coatings and dimensions.

710 C5 reflections

VP 708 Series

Lateral Tumble Stirrer

The Lateral Tumble Stirrer uses patented Magnetic Stir-Cylinder technology to mix large numbers of samples in microplates, racks of tubes, syringes, multiple large bottles, and carboys.

V&P Scientific, Inc. has invented and built the world’s first Lateral Tumble Stirrer (USA Patent #7,484,880, European Union Patent #1,736,235, and German Patent #60 2006 026 122.5).

Instead of tumbling a Stir Element in the vertical plane like our Vertical Tumble Stirrer, the Lateral Tumble Stirrer spins the stir elements by tumbling them laterally against a sidewall of the vessel. This spinning motion produces a vortex cone in the liquid and efficiently mixes the contents of the liquid. However, unlike the conventional horizontal stirrers which require a unique drive magnetic field under each vessel or well, the Lateral Vortex Tumble Stirrer because of its lateral wall tumbling action only requires one vertically placed drive magnetic cylinder to stir thousands of vessels or wells surrounding it. 

The Lateral Tumble Stirrer causes stir elements of all sizes and shapes to tumble laterally and create a vortex cone inside each well or vessel. The Lateral Tumble Stirrer will stir all types of microplates (24, 48, 96, 384, and even 1536 wells, and tubes, multiple large bottles, and even carboys.

Mix Microplates, Deep Well Microplates, Racks of Tubes, Syringes, multiple large Bottles, or Carboys

  1. Large and Strong Magnetic Cylinders
    • Allows use of Inexpensive Stir Elements
  2. High Throughput
    • Stirs 4,608 Wells at Once
  3. Run Multiple Experiments in Parallel
  4. Set Exact RPM’s
    • Enables Reproducible Results Day-to-Day
  5. Durable
    • Stir Continuously for Years Without Maintenance
  6. Stir Viscous Solutions
    • 6.6 Times More Viscous than Honey
  7. Can stir in any vessel regardless of the size or shape
  8. Can increase the aeration of microbial cultures
  9. CE compliant
    • Available in 110 and 220 volt versions

 











Using large and the most powerful permanent magnets manufactured, we make 2 different models of Lateral Tumble Stirrers to fit the many varied applications and requirements of our customers. Requirements for a long run time demand motors that are capable of working under high loads for extended periods. Please check with our knowledgeable staff to help you make the appropriate selection for your application. Our specialty is flexibility- let us know if you have a custom application today

We offer a large assortment of economical magnetic Stainless Steel Stir Elements from Stir StiXs to bars and dowels to fit into any well, tube, syringe large bottle or carboy. 

We also sell Alnico and Rare Earth Magnetic Stir Bars for more viscous solutions.

Check out the Stir Elements page for more information on coatings and dimensions.

vp707

VP 707 Series

Levitation Stirrer

The Levitation Stirrer uses patented Magnetic Levitation technology to mix large numbers of samples in deep well microplates (48, 96 and 384 microplates) (US patent #6,357,907; EU patent #1064988).

Instead of tumbling a Stir Element in a vessel the Levitation Stirrer uses a very strong magnetic dipole field to levitate stainless steel balls in deep well microplates. After several levitation passes the contents of the well are mixed from top to bottom. However, unlike the conventional horizontal stirrers which require a unique drive magnetic field under each well, the Levitation Stirrer because of its levitation action only requires one large magnetic dipole field to mix up to 4,608 wells (twelve 384 deep well microplates).

The Levitation Stirrer was initially made for Celera Genomics in the race to sequence the human genome. Celera Genomics needed to suspend replicated Human DNA released from bacteria at the bottom of 384 deep well microplates so the DNA could be read by the sequencer. Celera Genomics called the Levitation Stirrer an “enabling technology”.

Other applications include mixing very viscous materials such as paint, toothpaste and lotions with biocides, flavorings, or coloring agents to determine the proper concentration to use. Being able to perform mixing experiments in microplates allows one the ability to test many variables at the same time.

  1. Large and Strong Magnetic Dipoles
    • Allows use of Inexpensive Stir Elements (Ball Bearings)
  2. High Throughput
      • Stir 4,608 Wells at Once
  3. Heat (to 37°C) Stir and aerate
      • Culture bacterial and mammalian cells
  4. Run Multiple Experiments in Parallel
  5. Compatible with some Robotic Liquid Handlers
  6. Set Exact RPM’s
    • Enables Reproducible Results Day-to-Day
  7. Durable
      • Stir Continuously for Years Without Maintenance
  8. Stir Viscous Solutions
      • 6 Times More Viscous than Honey

The Carousel style which stirrers 12 deep well microplates (48, 96 and 384 wells) on a carousel plate and passes the microplates between a large and powerful NdFeB dipole magnet to raise magnetic stainless steel balls in the microplate and dropping them by gravity when they leave the magnetic field.

The second style of Levitation Stirrer only moves one deep well microplate back and forth on a linear shuttle and thus in and out of a powerful NdFeB dipole magnetic field to raise the balls and using gravity to drop the balls as they leave the magnetic field.

The third style of Levitation Stirrer also only moves one deep well microplate at a time but it does it by raising and lowering the microplate vertically in a larger and more powerful NdFeB dipole magnetic field so the magnetic stainless steel balls are both lifted up by the magnetic field and pulled down by the magnetic field. This is a very important ability when mixing very viscous fluids such as paint, lotions and toothpaste.

We offer an assortment of economical magnetic Stainless Steel ball bearings of different diameters. We also offer these same ball bearings that are coated with parylene when iron ions may interfere with the assay results. We also offer one Stainless Steel ball bearing that is coated with PTFE and another ALNICO round stir ball that is coated with PTFE. Our Alnico Magnetic Stir Balls are used for more viscous solutions.

Check out the  Stir Elements page for more information on coatings and dimensions.

vp706

VP 706 Series

Large Conventional Horizontal Stirrer

Our large conventional style stirrers are aimed at mixing industrial sized containers from carboys, barrels to 600 gallon containers. Our large NdFeB drive magnet is attached to Nema 34 quad stack servo motor and is capable to generating speeds of 1000 RPM.

Conventional Horizontal Magnetic Stirrers

The Conventional Horizontal Magnetic Stirrer technology has been around since 1944.  This system uses a motor to spin a drive magnet whose moving magnetic field connects with the magnetic field of a stir bar causing the stir bar to spin on a horizontal plane in a vessel above the drive magnet. V&P Scientific has improved that basic system.

Secrets Behind V&P Scientific’s Stirrus Technology:

  • The drive magnets in the V&P Scientific Stirrus machines use very large and very powerful (48 MGO) NdFeB rare earth magnets.  These are much larger and more powerful than the Alnico magnets (8 MGO) used in conventional horizontal stirrers.
  • This means that V&P Scientific Stirrus machines project their magnetic field farther and form a stronger attachment to the Stir elements thus allowing them to stir very viscous solutions.
  • V&P Scientific stir elements are made with powerful (48 to 52 MGO) NdFeB Rare Earth magnets and V&P is the only company to offer a full line of NdFeB stir elements to accommodate every vessel size.
  • V&P is also the only source for 48 and 52 MGO NdFeB Stir elements.
  • The extremely strong magnetic coupling/attraction between the V&P Scientific Stirrus drive magnet and the stir element, enables stirring at high speed and in a viscous material.
  • The motors in V&P Scientific Stirrus machines are very powerful and can maintain that power through both the low and the high speeds often required when mixing very viscous materials.
  • Even liquids as viscous as 90% honey are easily stirred producing impressive vortex cones. 
Applications of Conventional Magnetic (Horizontal) Stirring
  • There are many applications for conventional magnetic stirring ranging from simply mixing two or more compounds together, or dissolving one compound into a liquid, or mixing to speed a chemical reaction.
  • Many of the customers who are using our Stirrus Machines (VP 706 series) do so because they need to have the power to be able to mix very viscous liquids at both slow and high speeds.
  • Another need is to be able to stir vessels as large as 600 gallons.
  • Another capability of the Stirrus Machines arises from our ability to manufacture stir elements specifically made to couple strongly with the Stirrus’ drive magnetic field.
  • We also make stir elements for our Stirrus Machines whose physical shape can be changed to match the application or shape of the vessel.
  • One of the unusual applications is the use of Stirrus Machines to macerate chicken embryo tissue into single-cell preparations to seed large tissue culture bottles for vaccine production.
  • Other customers have used the Stirrus Machines to mix slurries.
  1. Can stir large volumes of viscous material
  2. Can support the weight of large containers
  3. Can spin at high and low speed
  4. Drive Magnet design enhances coupling to Stir Element

The MidiStirrus is capable of stirring vessels up to carboy size vessels

The KiloStirrus is capable of stirring vessels up to 600 gallons in size.

We offer a large assortment of ALNICO, Samarium Cobalt and Neodymium Iron Boron Stir Elements from stir bars to H shapes and suspended stir elements to fit into any Carboy or large vessel. Check out the Stir Elements page for more information on ALNICO and Rare Earth Magnetic Stir Bars for more viscous solutions

Feedback for the Tumble Stirrers is this: enabling technology. I am now enabled to create reaction blocks and customize them to robotic systems with great ease.  Why? The narrow deck height of the Tumble stirrers (even my larger strength units) combined with flexibility of alignment for the stirrers to the reaction vessels means I can use them almost anywhere.

The fact that I can use the same stirrer deck with any type/size of reactor simply by changing a mounting plate atop the stirrer deck gives me the kind of flexibility I require for parallel synthetic development of processes. That flexibility enables access to a wider range of projects (smaller scale) and a wider range of reaction conditions. I get more reactions per gram of starting material for evaluation of more diverse conditions, and I generate results faster because I run more of the experiments in parallel using the same amount of starting material. In one word, Tumble Stirring is enabling.

Oh, and the quality of agitation is fantastic for liquid-liquid mixes, slurries, or even oil suspensions.

Thanks again for this terrific product.

D____.”

— Investigator in Process R&D from a large pharmaceutical company​

Additonal Offerings

OEM Cylinders

Contact us if you wish to integrate our patented stirring technology into your liquid handling system. We can tailor our magnets to fit your application

Stirrer Accessories

We have many different accessories associated with our VP Stirrer Series. See the full complement below.

Category
Container Used

Stirrer Products

Showing 37–44 of 44 results

Hydrophobic Coated vs Non-Coated Pin Transfers

Solid Pin Delivery Data For Aqueous Solutions In 96 Format With Uncoated And /Ah Hydrophobic Coated Pins
PinDescriptionnl TransferredCV%
0.229 mm diameter (FP9)Total PinUncoated7.412.4
Hydrophobic7.465.4
0.229 mm diameter (FP9)Hanging DropUncoatedN/AN/A
Hydrophobic2.093.8
0.457 mm diameter (FP1)Total PinUncoated33.483.2
Hydrophobic28.177.5
0.457 mm diameter (FP1)Hanging DropUncoated16.964.5
Hydrophobic8.510.8
0.787 mm diameter (FP3)Total PinUncoated87.323.9
Hydrophobic77.43.9
0.787 mm diameter (FP3)Hanging DropUncoated48.771.2
Hydrophobic43.059.4
1.19 mm diameter  (VP 409 & VP 386)Total PinUncoated247.222.8
Hydrophobic192.672.6
1.19 mm diameter (VP 409 & VP 386)Hanging DropUncoated76.351.6
Hydrophobic108.42.8
1.58 mm diameter (VP 408 & VP 384)Total PinUncoated273.54.6
Hydrophobic259.253.1
1.58 mm diameter (VP 408 & VP 384)Hanging DropUncoated201.935
Hydrophobic170.047.5

Transfer Of Horseradish Peroxidase In Tris Buffered Saline With Pin Tools

Conclusion

Coating pins will reduce the total amount of liquid transferred and also reduce the amount of non-specific binding to the stainless-steel pins. If the substance you are transferring has high non-specific binding this will be an important factor in selecting your pins.

Slot Pin Delivery Data For Aqueous Solutions In 96 Format With Uncoated And /Ah Hydrophobic Coated Pin
PinDescriptionnl TransferredCV%
0.229 mm diameter (FP9)Total PinUncoated7.412.4
Hydrophobic7.465.4
0.229 mm diameter (FP9)Hanging DropUncoatedN/AN/A
Hydrophobic2.093.8
0.457 mm diameter (FP1)Total PinUncoated33.483.2
Hydrophobic28.177.5
0.457 mm diameter (FP1)Hanging DropUncoated16.964.5
Hydrophobic8.510.8
0.787 mm diameter (FP3)Total PinUncoated87.323.9
Hydrophobic77.43.9
0.787 mm diameter (FP3)Hanging DropUncoated48.771.2
Hydrophobic43.059.4
1.19 mm diameter  (VP 409 & VP 386)Total PinUncoated247.222.8
Hydrophobic192.672.6
1.19 mm diameter (VP 409 & VP 386)Hanging DropUncoated76.351.6
Hydrophobic108.42.8
1.58 mm diameter (VP 408 & VP 384)Total PinUncoated273.54.6
Hydrophobic259.253.1
1.58 mm diameter (VP 408 & VP 384)Hanging DropUncoated201.935
Hydrophobic170.047.5

Transfer Of Horseradish Peroxidase In Tris Buffered Saline With Pin Tools

Conclusion

Although the slots in the pin are a precise volume, the liquid that is transferred is usually more. The reason for this is due to the surface tension of the liquid causing the liquid in the slot to “bow out” thus increasing the volume of the liquid in the slot. If is important for you to transfer exactly a certain volume we can make custom slots to match the surface tension characteristics of your liquid

Liquid Surface Tension

Effect Of DNA Or BSA Concentration On Slot Pin Transfers Of Uncoated And Hydrophobic Coated Pins (FP3CS500)
Solvent/SampleConcentrationCV%nl FITC TransferredCV%nl FITC Transferred
UncoatedUncoatedHydrophobic CoatedHydrophobic Coated
DMSO (-)08.1353.427.5298.72
DMSO + DNA (mg/ml)0.56.6497.216.6435.86
0.259432.494.1391.93
0.1258.9363.640.9344.75
0.06252.3381.862331.68
0.03131.5378.034.4331.71
0.01561.2357.521.4329.03
Tris (-)04.9577.317.2493.53
Tris + DNA (mg/ml)0.54.5540.531.1477.5
0.254.6518.216.1456.75
0.12515.8583.254.1438.82
0.06254.2551.173.1433.69
0.03134.4536.662.3458.37
0.01562.9528.531.2441.1
Tris + BSA (%)45.4462.1311409.27
14452.862.7426.58
0.2511.7456.451.3408.72
0.06251.1445.226.5393.07
0.01563.7462.853.9430.2
0.00391.5493.542.2437.29
0.0012.9504.250.7475.96
Conclusions

1. Increasing the concentration of DNA (sheared salmon sperm) to .25 mg/ml significantly increases the volume of DMSO liquid transferred for both coated and uncoated FP3S500 Slot Pins.
2. Increasing the concentration of DNA does not significantly increase the volume of Tris buffer (aqueous) transferred by both coated and uncoated FP3S500 Slot Pins.
3. Increasing the concentration of BSA (Bovine Serum Albumin) significantly decreases the volume of Tris buffer transferred by both coated and uncoated FP3S500 Slot Pins.
4. Hydrophobic coated FP3S500 Slot Pins transferred less DMSO – DNA and less Tris DNA and less Tris BSA than the uncoated FP3S500 Slot Pins.
5. Both coated and uncoated FP3S500 pins transfer significantly more aqueous solution than DMSO.

Effect Of DNA Or BSA Concentration On Slot Pin Transfers Of Uncoated And Hydrophobic Coated Pins (FP1CS50)
Solvent/SampleConcentrationCV%nl FITC TransferredCV%nl FITC Transferred
UncoatedUncoatedHydrophobic CoatedHydrophobic Coated
DMSO (-)04.249.382.149.31
DMSO + DNA (mg/ml)0.54.951.242.656.79
0.251.750.21.249.53
0.1251.551.272.349.77
0.06252.249.344.148.19
0.03131.249.030.250.23
0.01562.445.91.446.64
Tris (-)02.689.512.991.34
Tris + DNA (mg/ml)0.5777.110.684.62
0.253.982.221.684.89
0.1253.985.42185.08
0.06251.585.362.885.03
0.0313284.52388.19
0.01562.682.922.883.2
Conclusions

1. In contrast to the FP3S500 data, increasing the concentration of DNA to .25 mg/ml does not significantly increase the volume of DMSO liquid transferred for both coated and uncoated FP1S50 Slot Pins.
2. Increasing the concentration of DNA does not significantly increase the volume of Tris buffer (aqueous) transferred by both coated and uncoated FP1S50 Slot Pins.
3. In contrast to the FP3S500 data, FP1S50 coated pins transferred about the same volume of DNA at all concentrations as did uncoated pins.
4. Both coated and uncoated FP1S50 pins transfer significantly more aqueous solution than DMSO.
5. The differences between the FP3S500 and the FP1S50 pin may be due to the different pin diameter’s effect on contact angle and therefore on the “wetting” of the pin. See the diagram on the link to / ah energy system.

PinDescriptionnl TransferredCV%
0.229 mm diameter (FP9)Total PinUncoated7.412.4
Hydrophobic7.465.4
0.229 mm diameter (FP9)Hanging DropUncoatedN/AN/A
Hydrophobic2.093.8
0.457 mm diameter (FP1)Total PinUncoated33.483.2
Hydrophobic28.177.5
0.457 mm diameter (FP1)Hanging DropUncoated16.964.5
Hydrophobic8.510.8
0.787 mm diameter (FP3)Total PinUncoated87.323.9
Hydrophobic77.43.9
0.787 mm diameter (FP3)Hanging DropUncoated48.771.2
Hydrophobic43.059.4
1.19 mm diameter  (VP 409 & VP 386)Total PinUncoated247.222.8
Hydrophobic192.672.6
1.19 mm diameter (VP 409 & VP 386)Hanging DropUncoated76.351.6
Hydrophobic108.42.8
1.58 mm diameter (VP 408 & VP 384)Total PinUncoated273.54.6
Hydrophobic259.253.1
1.58 mm diameter (VP 408 & VP 384)Hanging DropUncoated201.935
Hydrophobic170.047.5

Aqueous Transfer with Solid Pins

Hydrophobic coating pins will reduce the total amount of aqueous HRP liquid transferred and also reduce the amount of non-specific binding to the stainless-steel pins. If the substance you are transferring has high non-specific binding this will be an important factor in selecting your pins.

 

Pin diameter also has an effect on the degree of reduction of liquid transfer with hydrophobic coating as the smaller the diameter the less the reduction of transfer. This is most likely due to the curvature of the pin affecting the wetting contact angle

PinDescriptionnl TransferredCV%
0.457 mm diameter (FP1)6 nl SlotTotal Pin*Uncoated25.610.8
HydrophobicN/AN/A
10 nl SlotTotal Pin*Uncoated23.366.1
Hydrophobic25.856.9
50 nl SlotTotal Pin*Uncoated67.832.5
HydrophobicN/AN/A
0.787 mm diameter (FP3)  100 nl SlotTotal Pin*Uncoated180.327.2
Hydrophobic205.845.5
200 nl SlotTotal Pin*Uncoated277.824.9
Hydrophobic287.33.8
500 nl SlotTotal Pin*Uncoated581.165.2
Hydrophobic555.693

DMSO Transfer with Slot Pins

Hydrophobic coating pins will slightly increase the total amount of DMSO FITC liquid transferred.

PinDescriptionnl TransferredCV%
0.787 mm diameter (FP3)    100 nl Slot Total Pin, Including SlotUncoated195.691.6
Hydrophobic170.22.9
0.787 mm diameter (FP3)  100 nl Slot, Slot OnlyUncoated149.674.9
Hydrophobic129.617.6
0.787 mm diameter (FP3)200 nl Slot Total Pin, Including SlotUncoated269.771.9
Hydrophobic228.6217.1
0.787 mm diameter (FP3)200 nl Slot, Slot OnlyUncoated237.528.9
Hydrophobic186.95.9

Aqueous Transfer with Slot Pins

Although the slots in the pin are a precise volume, the liquid that is transferred is usually more because of the volume carried on the sides of the pins. 

As seen with other aqueous data the amount transferred on hydrophobic coated Slot pins is less than on uncoated Solid or Slot pins. Thus Hydrophobic coating has the most effect on aqueous transfers.

Withdrawl Speeds Impact on Volume Transfer

Solid Pins More affected by Source Plate Volume

Volume Transferred For FP1 Pins (Uncoated) In 96 And 384 Formats
Volume Transferred For FP3 Pins (Uncoated) In 96 And 384 Formats

Note: Same volume (200ul for 96 Format and 74 ul for 384 Format) in recipient plates and same pin withdrawal speed for all pins. Changes to pin withdrawal speed or volume in the source plate can result in different volumes being transferred.

Transfer volumes should always be confirmed by customers for their assay conditions and automated system.

Aqueous Solutions Pin Transfer Volumes Ranges

Aqueous Solutions on Uncoated Pins in 96 Format Microplates(1)
Pin TypePin Diameter(mm)Shape96 Format Low Range(nL)²96 Format High Range(nL)²
FP90.229Solid1339
FP80.356Solid1537
FP10.457Solid2261
FP1S60.4576nL Slot3467
FP1S100.45710nL Slot3974
FP1S500.45750nL Slot90124
FP30.787Solid93213
FP3S1000.787100nL Slot213334
FP3S2000.787200nL Slot311449
FP3S5000.787500nL Slot515671
FP40.914Solid126289
Footnotes: (1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well
Aqueous Solutions on Hydrophobic Pins in 96 Format Microplates(1)
Pin TypePin Diameter(mm)Shape96 Format Low Range(nL)²96 Format High Range(nL)²
FP90.229Solid1338
FP80.356Solid
FP10.457Solid2360
FP1S60.4576nL Slot3367
FP1S100.45710nL Slot4075
FP1S500.45750nL Slot86119
FP30.787Solid76209
FP3S1000.787100nL Slot188324
FP3S2000.787200nL Slot288436
FP3S5000.787500nL Slot473649
FP40.914Solid
Footnotes: (1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well
Aqueous Solution on E-Clip, Uncoated Pins(1)
Pin TypePin Diameter(mm)ShapeLow Range(nL)²High Range(nL)²
FP1.58Solid Pointed175594
FPS.51.58500nL Slot524962
FPS1.581000nL Slot10561476
FPS21.582000nL Slot17392174
FPS51.585000nL Slot51504953
FP61.58Solid Flat465960
FP6S.51.58500nL Slot9341445
FP6S1.581000nL Slot13961930
FP6S21.582000nL Slot20722637
FP6S51.585000nL Slot48204693
Footnotes:(1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well for 96 Format and 75ul source plate volume per well for 384 Format

DMSO Pin Transfer Volume Range Charts

Uncoated Pins in 96 and 384 Format Microplates(1)
Pin TypePin Diameter(mm)Shape96 Format Low Range(nL)²96 Format High Range(nL)²384 Format Low Range(nL)³384 Format High Range(nL)³
FP90.229Solid41038
FP80.35Solid1326618
FP10.457Solid18431131
FP1S60.4576nL Slot24491534
FP1S100.45710nL Slot30542140
FP1S200.45720nL Slot37612746
FP1S300.45730nL Slot46683554
FP1S400.45740nL Slot57784563
FP1S500.45750nL Slot70905675
FP30.787Solid671392979
FP40.91Solid941973498
FP3S1000.787100nL Slot175241114163
FP3S2000.787200nL Slot280332203250
FP3S5000.787500nL Slot535559427464
FP4S10000.911000nL Slot9401011704800
FP4S20000.912000nL Slot1518160812771362
Footnotes: (1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well (3) 75ul source plate volume per well
Hydrophobic-coated Pins in 96 and 384 Format Microplates(1)
Pin TypePin Diameter (mm)Shape96 Format Low Range(nL)²96 Format High Range(nL)²384 Format Low Range(nL)³384 Format High Range(nL)³
FP9H0.229Solid41038
FP8H0.35Solid924617
FP1H0.457Solid1539927
FP1S6H0.4576nL Slot23491432
FP1S10H0.45710nL Slot29532038
FP1S20H0.45720nL Slot35592643
FP1S30H0.45730nL Slot47693553
FP1S40H0.45740nL Slot54754158
FP1S50H0.45750nL Slot69905773
FP3H0.787Solid671342776
FP4H0.91Solid9518932102
FP3S100H0.787100nL Slot170227108164
FP3S200H0.787200nL Slot266320190239
FP3S500H0.787500nL Slot520542416456
FP4S1000H0.911000nL Slot9321000741805
FP4S2000H0.912000nL Slot1571163813511423
Footnotes: (1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well (3) 75ul source plate volume per well
E-Clip, Uncoated Pins, for 96 and 384 Format Microplates(1)
Pin TypeDiameter (mm)Shape96 Format Low Range(nL)²96 Format High Range(nL)²384 Format Low Range(nL)³384 Format High Range(nL)³
FP1.58Solid Pointed147411168395
FPS.51.58500nL Slot442704631843
FPS1.581000nL Slot893113013431498
FPS21.582000nL Slot1911203826072767
FPS51.585000nL Slot3908429651805253
FP61.58Solid Flat323674154398
FP6S.51.58500nL Slot73410428551053
FP6S1.581000nL Slot1210150016381717
FP6S21.582000nL Slot2299238427873068
FP6S51.585000nL Slot4329465652375245
Footnotes:(1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well (3) 75ul source plate volume per well