VP 750AL-2A
VP 750AL-2A (1)
VP 750AL-2A
VP 750AL-2A (1)
VP 750AL-2A

VP 750AL-2A

BUBBLE PADDLE RESERVOIR, Tumble Powered, 96 Channel, Aluminum, Hydrophilic Coated, Flat Bottom Reservoir, Mates to 710C Series

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BUBBLE PADDLE RESERVOIR, Tumble Powered, 96 Channel, Aluminum, Hydrophilic Coated, Flat Bottom Reservoir, Holds Either  VP 750-ECON-6AP Polycarbonate Bubble Paddle Frame (6 Bubble Paddles, Parylene Coated, 7 Bubbles/Paddle With PTFE Bearings) or VP 750-ECON-6P Frame (4 Bubble Paddles, Parylene Coated, 11 Bubbles/Paddle With PTFE Bearings), Mixes 280mL, SLAS Footprint, 127.7mm Length, 85.5mm Width, 50mm Height

 

Drive System

Tumble Stirrer
Pipette Head Format

96
Material

Aluminum, Polycarbonate
No of Bubble Paddles

4, 6
Bubbles/Paddle

11, 7
Encapsulation/Coating

Parylene
Volume

280mL
SLAS Base

Yes
Particulate Tumble Stirrer Suspension Systems in Reservoirs – Many Choices

V&P has developed over 30 different reservoir suspension systems for pipetting uniformly suspended beads, cells, particles, and resins into microplates of nearly every description.  We have refined, perfected, and consolidated that list down to the best 22.  These reservoir suspension systems are powered by one of our 15 different Vertical Tumble Stirrers.

Uniformity of Suspension and Low Dead Volumes

Keeping magnetic beads, assay beads, SPA beads, glass beads, resins other particulates, and cells in homogeneous suspension while they are being pipetted to microplate wells is very important if reproducible results are to be obtained. It is also important to do this with the smallest dead volume possible when the reagents are very expensive. Although one might think you cannot use magnets to stir and suspend magnetic beads, V&P has found a way to uniformly suspend magnetic beads with our parylene-coated suspension systems and Magnetic Vertical Tumble Stirrers.

The unique V&P Scientific Bubble Paddle Reservoir suspension systems (US Patent #6,461,034 & European Union Patent #1,314,471) provide a very simple and inexpensive way of accomplishing this goal. Whether you are using a 4, 8, 16, 24, 96, or 384 pipetting head, there is a suspension reservoir with a bubble spacing that allows a pipette tip to be inserted in the gap between bubbles. The unique contours machined into the reservoir coupled with the contours of the Bubble Paddle design results in very vigorous stirring and homogeneous suspension of the particulate contents in the reservoir. The unique feature of the Bubble Paddle Reservoirs is their exclusive design that eliminates “fall out zones” where particulates fall out of solution that is so prevalent with other mixing reservoirs. Also if dead volume is not an issue in your assay you can use our economical VP 756B-4PPP suspension reservoir powered by a VP 710D3 Tumble Stirrer in conjunction with a 4, 8, or 16 pipette head for a low-cost reservoir suspension system.

Match the Particulate and Application to the Appropriate Suspension Reservoir System

There are many characteristics of your material that will affect where and how they are suspended:

  • Size of the material – is it larger than the paddle/reservoir contour gap
  • Density of the material
  • Fragility of the material
  • Speed at which it can be stirred without breakage
  • Temperature sensitivity of the material
  • Light sensitivity of the material
  • Does the reservoir need to be continuously fed?

 

All of the features above are very important; choose your suspension system wisely before you launch a new assay protocol. We have dealt with it all in the last 30 years. With our large selection of Suspension Reservoirs Systems, we can help you make the best decision for your application.  We are happy to share our knowledge with you.

Keeping Magnetic Beads in Suspension with Magnetism

We discovered that by using parylene-coated bubble paddles with Vertical Tumble Stirrers, we could easily keep magnetic beads in uniform suspension and the magnetic beads would not remain attracted to the parylene-coated bubble paddles, or stay on the bottom of the reservoir once the paddles begin to move.  This greatly expands the number of systems possible for a magnetic bead application, furthermore, it simplifies and reduces the cost of the magnetic bead suspension systems:

If economy and uniformity of suspension are important to you, we have solutions for you.

Advantages of V&P Scientific’s Tumble Stirrer Suspension Reservoir Systems

V&P’s patented contours in the bottom of the reservoir which match the contours of the Bubble Paddles produce a vigorous upwelling current in the reservoirs to keep particulates in uniform suspension.

  • The Bubble Paddles are contoured so pipette tips will fit in the gaps between the bubbles and not interfere with spinning bubble paddles
  • V&P’s patented contours in the bottom design also eliminate “fall out zones” where particulates settle out in other mixing reservoirs
  • Designed to fit on robot deck features to facilitate precise x and y matching to the robot pipetting system
  • Designed to be taken off the deck and replaced in the exact same position
  • Greatest variety of Suspension Reservoirs Systems for 1, 4, 8, 12, 16, 24, 96, and 384 pipettors
  • Multiple versions of Suspension Reservoirs Systems for different volumes and applications
  • Available in chemical resistant materials (PTFE, Delrin, Polypropylene)
  • Available in light blocking materials (Black Delrin, Aluminum)
  • Available in autoclavable or chemically sterilizable materials
  • Available in Aluminum versions for heating or chilling the contents of the reservoir
  • Low dead volume designs to conserve expensive reagents
  • Available in Hydrophilic coatings to reduce dead volume loss, prevents droplet formation, and reduces air bubble formation in crevices
  • Available in versions to suspend particulates in multiple unique reservoirs simultaneously
  • Capable of continuous refilling for long run times
  • Capable of mixing even solutions as viscous as honey (15,000 centistokes)
  • Custom Suspension Reservoir Systems are not a problem
  • Flexibility is our specialty

 

Accessories

See Vertical Tumble Stirrers that power our Suspension Reservoir Systems.

See our other accessories for Suspension Reservoirs.

V&P Scientific’s Patented  Suspension Reservoir System Applications

Keep any non-magnetic particulate in homogeneous suspension for pipetting of equal particle quantities into microplates or vials.  There’s an endless amount of possible applications.
  • Beads
  • Magnetic Beads
  • Glass Beads (YSi Beads)
  • Gold Beads
  • Nano Beads
  • Cellulose Nanobeads
  • Sepharose Beads
  • Agarose Beads
  • Latex Beads
  • Resins
  • Polymeric Microbeads
  • Mammalian cells
  • Bacterial cells
  • Fungal cells
  • Viruses
  • Quantum-dot nanocrystals

Our Suspension Reservoir Systems accommodate many pipetting robots and can also be used on the bench top with manual with either single, 8 or 12 channel pipets.
  • 1
  • 4
  • 8
  • 12
  • 16
  • 24
  • 96
  • 384

Our specialized Suspension Reservoir Systems can be used in a variety of different environments, and with unique liquids.
  • Sterile liquids
  • Heated liquids
  • Cooled liquids
  • Light sensitive reagents
  • Organic solvents
  • Viscous liquids
  • Fragile particulates

V&P Scientific’s Hydrophilic Coating

Our Hydrophilic coating is a very hard, abrasion resistant, and chemical resistant surface for metals and ceramics. It resists all acids except hydrofluoric, all bases, and all polar and non-polar solvents. It exhibits good thermal conductivity from lattice phonon conduction from its strong bonding and because it is comprised of comparatively light atomic species. It also is a high dielectric surface from the lack of conduction of free electrons across a large electronic bandgap. The effective coating on metals for corrosion resistance is nearly transparent and thin, in the range of 750 to 1200 Angstroms. Effective coatings for abrasion resistance are deeper, in the range of 1500-2000 Angstroms, and tend to be more opaque. The maximum service temperature in an oxidizing atmosphere is 120°C and will offer protection in a reducing or an inert atmosphere to 201°C. The coating is applied in an environmentally benign process, and potentially damaging plasma energy is not utilized. It is an FDA-approved material for full-food contact, surgical instruments, and for surgical implantation. It is can be autoclaved and may be chemically sterilized.

Advantages of Hydrophilic Coated Reservoirs

The hydrophilic coating on our aluminum reservoirs maximizes the recovery of the reagents used in them.  The hydrophilic coating prevents droplet formation and causes the reagent to collect in the dimpled area.  We also facilitate this collection of reagent in the dimples by bringing the walls into the dimpled area and then rounding that corner to prevent loss of liquid in the corner.  The Polypropylene, Delrin, and PTFE reservoirs described above all are hydrophobic and leave droplets of reagents in the flat areas and in the corners and therefore have larger “dead volumes”.

Another advantage of the aluminum reservoirs with control wells is that we can make the separating walls thinner with aluminum than with the plastics (Polypropylene, Delrin, and PTFE) 0.7 mm thick versus 1.3 mm thick, thus eliminating potential problems with some brands of pipet tips hanging up on the separating walls when they near the bottom of the reservoir.

A third advantage of the hydrophilic coated aluminum reservoirs is that air bubbles are not trapped in the dimples at the bottom.  Just pour a little reagent in, swirl and the dimples fill with your liquid, and the air is not trapped in the dimple.  This is not the case with Polypropylene, Delrin, and PTFE where the air in the dimples has to be removed (dislodged by pipet tip) or by centrifugation.

Our hydrophilic coating is not affected by 30% H2O2, salt, all acids except hydrofluoric acid, all bases, all polar and non-polar solvents, and potassium ferrocyanide. It also can be autoclaved and chemically sterilized.

130B Tumble Bubble Paddle Reservoir

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Since its founding in 1979 by Dr. Patrick Cleveland and his wife Victoria, V&P’s goal has been to provide the best engineered and most innovative research equipment available.

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Hydrophobic Coated vs Non-Coated Pin Transfers

Solid Pin Delivery Data For Aqueous Solutions In 96 Format With Uncoated And /Ah Hydrophobic Coated Pins
PinDescriptionnl TransferredCV%
0.229 mm diameter (FP9)Total PinUncoated7.412.4
Hydrophobic7.465.4
0.229 mm diameter (FP9)Hanging DropUncoatedN/AN/A
Hydrophobic2.093.8
0.457 mm diameter (FP1)Total PinUncoated33.483.2
Hydrophobic28.177.5
0.457 mm diameter (FP1)Hanging DropUncoated16.964.5
Hydrophobic8.510.8
0.787 mm diameter (FP3)Total PinUncoated87.323.9
Hydrophobic77.43.9
0.787 mm diameter (FP3)Hanging DropUncoated48.771.2
Hydrophobic43.059.4
1.19 mm diameter  (VP 409 & VP 386)Total PinUncoated247.222.8
Hydrophobic192.672.6
1.19 mm diameter (VP 409 & VP 386)Hanging DropUncoated76.351.6
Hydrophobic108.42.8
1.58 mm diameter (VP 408 & VP 384)Total PinUncoated273.54.6
Hydrophobic259.253.1
1.58 mm diameter (VP 408 & VP 384)Hanging DropUncoated201.935
Hydrophobic170.047.5

Transfer Of Horseradish Peroxidase In Tris Buffered Saline With Pin Tools

Conclusion

Coating pins will reduce the total amount of liquid transferred and also reduce the amount of non-specific binding to the stainless-steel pins. If the substance you are transferring has high non-specific binding this will be an important factor in selecting your pins.

Slot Pin Delivery Data For Aqueous Solutions In 96 Format With Uncoated And /Ah Hydrophobic Coated Pin
PinDescriptionnl TransferredCV%
0.229 mm diameter (FP9)Total PinUncoated7.412.4
Hydrophobic7.465.4
0.229 mm diameter (FP9)Hanging DropUncoatedN/AN/A
Hydrophobic2.093.8
0.457 mm diameter (FP1)Total PinUncoated33.483.2
Hydrophobic28.177.5
0.457 mm diameter (FP1)Hanging DropUncoated16.964.5
Hydrophobic8.510.8
0.787 mm diameter (FP3)Total PinUncoated87.323.9
Hydrophobic77.43.9
0.787 mm diameter (FP3)Hanging DropUncoated48.771.2
Hydrophobic43.059.4
1.19 mm diameter  (VP 409 & VP 386)Total PinUncoated247.222.8
Hydrophobic192.672.6
1.19 mm diameter (VP 409 & VP 386)Hanging DropUncoated76.351.6
Hydrophobic108.42.8
1.58 mm diameter (VP 408 & VP 384)Total PinUncoated273.54.6
Hydrophobic259.253.1
1.58 mm diameter (VP 408 & VP 384)Hanging DropUncoated201.935
Hydrophobic170.047.5

Transfer Of Horseradish Peroxidase In Tris Buffered Saline With Pin Tools

Conclusion

Although the slots in the pin are a precise volume, the liquid that is transferred is usually more. The reason for this is due to the surface tension of the liquid causing the liquid in the slot to “bow out” thus increasing the volume of the liquid in the slot. If is important for you to transfer exactly a certain volume we can make custom slots to match the surface tension characteristics of your liquid

Liquid Surface Tension

Effect Of DNA Or BSA Concentration On Slot Pin Transfers Of Uncoated And Hydrophobic Coated Pins (FP3CS500)
Solvent/SampleConcentrationCV%nl FITC TransferredCV%nl FITC Transferred
UncoatedUncoatedHydrophobic CoatedHydrophobic Coated
DMSO (-)08.1353.427.5298.72
DMSO + DNA (mg/ml)0.56.6497.216.6435.86
0.259432.494.1391.93
0.1258.9363.640.9344.75
0.06252.3381.862331.68
0.03131.5378.034.4331.71
0.01561.2357.521.4329.03
Tris (-)04.9577.317.2493.53
Tris + DNA (mg/ml)0.54.5540.531.1477.5
0.254.6518.216.1456.75
0.12515.8583.254.1438.82
0.06254.2551.173.1433.69
0.03134.4536.662.3458.37
0.01562.9528.531.2441.1
Tris + BSA (%)45.4462.1311409.27
14452.862.7426.58
0.2511.7456.451.3408.72
0.06251.1445.226.5393.07
0.01563.7462.853.9430.2
0.00391.5493.542.2437.29
0.0012.9504.250.7475.96
Conclusions

1. Increasing the concentration of DNA (sheared salmon sperm) to .25 mg/ml significantly increases the volume of DMSO liquid transferred for both coated and uncoated FP3S500 Slot Pins.
2. Increasing the concentration of DNA does not significantly increase the volume of Tris buffer (aqueous) transferred by both coated and uncoated FP3S500 Slot Pins.
3. Increasing the concentration of BSA (Bovine Serum Albumin) significantly decreases the volume of Tris buffer transferred by both coated and uncoated FP3S500 Slot Pins.
4. Hydrophobic coated FP3S500 Slot Pins transferred less DMSO – DNA and less Tris DNA and less Tris BSA than the uncoated FP3S500 Slot Pins.
5. Both coated and uncoated FP3S500 pins transfer significantly more aqueous solution than DMSO.

Effect Of DNA Or BSA Concentration On Slot Pin Transfers Of Uncoated And Hydrophobic Coated Pins (FP1CS50)
Solvent/SampleConcentrationCV%nl FITC TransferredCV%nl FITC Transferred
UncoatedUncoatedHydrophobic CoatedHydrophobic Coated
DMSO (-)04.249.382.149.31
DMSO + DNA (mg/ml)0.54.951.242.656.79
0.251.750.21.249.53
0.1251.551.272.349.77
0.06252.249.344.148.19
0.03131.249.030.250.23
0.01562.445.91.446.64
Tris (-)02.689.512.991.34
Tris + DNA (mg/ml)0.5777.110.684.62
0.253.982.221.684.89
0.1253.985.42185.08
0.06251.585.362.885.03
0.0313284.52388.19
0.01562.682.922.883.2
Conclusions

1. In contrast to the FP3S500 data, increasing the concentration of DNA to .25 mg/ml does not significantly increase the volume of DMSO liquid transferred for both coated and uncoated FP1S50 Slot Pins.
2. Increasing the concentration of DNA does not significantly increase the volume of Tris buffer (aqueous) transferred by both coated and uncoated FP1S50 Slot Pins.
3. In contrast to the FP3S500 data, FP1S50 coated pins transferred about the same volume of DNA at all concentrations as did uncoated pins.
4. Both coated and uncoated FP1S50 pins transfer significantly more aqueous solution than DMSO.
5. The differences between the FP3S500 and the FP1S50 pin may be due to the different pin diameter’s effect on contact angle and therefore on the “wetting” of the pin. See the diagram on the link to / ah energy system.

PinDescriptionnl TransferredCV%
0.229 mm diameter (FP9)Total PinUncoated7.412.4
Hydrophobic7.465.4
0.229 mm diameter (FP9)Hanging DropUncoatedN/AN/A
Hydrophobic2.093.8
0.457 mm diameter (FP1)Total PinUncoated33.483.2
Hydrophobic28.177.5
0.457 mm diameter (FP1)Hanging DropUncoated16.964.5
Hydrophobic8.510.8
0.787 mm diameter (FP3)Total PinUncoated87.323.9
Hydrophobic77.43.9
0.787 mm diameter (FP3)Hanging DropUncoated48.771.2
Hydrophobic43.059.4
1.19 mm diameter  (VP 409 & VP 386)Total PinUncoated247.222.8
Hydrophobic192.672.6
1.19 mm diameter (VP 409 & VP 386)Hanging DropUncoated76.351.6
Hydrophobic108.42.8
1.58 mm diameter (VP 408 & VP 384)Total PinUncoated273.54.6
Hydrophobic259.253.1
1.58 mm diameter (VP 408 & VP 384)Hanging DropUncoated201.935
Hydrophobic170.047.5

Aqueous Transfer with Solid Pins

Hydrophobic coating pins will reduce the total amount of aqueous HRP liquid transferred and also reduce the amount of non-specific binding to the stainless-steel pins. If the substance you are transferring has high non-specific binding this will be an important factor in selecting your pins.

 

Pin diameter also has an effect on the degree of reduction of liquid transfer with hydrophobic coating as the smaller the diameter the less the reduction of transfer. This is most likely due to the curvature of the pin affecting the wetting contact angle

PinDescriptionnl TransferredCV%
0.457 mm diameter (FP1)6 nl SlotTotal Pin*Uncoated25.610.8
HydrophobicN/AN/A
10 nl SlotTotal Pin*Uncoated23.366.1
Hydrophobic25.856.9
50 nl SlotTotal Pin*Uncoated67.832.5
HydrophobicN/AN/A
0.787 mm diameter (FP3)  100 nl SlotTotal Pin*Uncoated180.327.2
Hydrophobic205.845.5
200 nl SlotTotal Pin*Uncoated277.824.9
Hydrophobic287.33.8
500 nl SlotTotal Pin*Uncoated581.165.2
Hydrophobic555.693

DMSO Transfer with Slot Pins

Hydrophobic coating pins will slightly increase the total amount of DMSO FITC liquid transferred.

PinDescriptionnl TransferredCV%
0.787 mm diameter (FP3)    100 nl Slot Total Pin, Including SlotUncoated195.691.6
Hydrophobic170.22.9
0.787 mm diameter (FP3)  100 nl Slot, Slot OnlyUncoated149.674.9
Hydrophobic129.617.6
0.787 mm diameter (FP3)200 nl Slot Total Pin, Including SlotUncoated269.771.9
Hydrophobic228.6217.1
0.787 mm diameter (FP3)200 nl Slot, Slot OnlyUncoated237.528.9
Hydrophobic186.95.9

Aqueous Transfer with Slot Pins

Although the slots in the pin are a precise volume, the liquid that is transferred is usually more because of the volume carried on the sides of the pins. 

As seen with other aqueous data the amount transferred on hydrophobic coated Slot pins is less than on uncoated Solid or Slot pins. Thus Hydrophobic coating has the most effect on aqueous transfers.

Withdrawl Speeds Impact on Volume Transfer

Solid Pins More affected by Source Plate Volume

Volume Transferred For FP1 Pins (Uncoated) In 96 And 384 Formats
Volume Transferred For FP3 Pins (Uncoated) In 96 And 384 Formats

Note: Same volume (200ul for 96 Format and 74 ul for 384 Format) in recipient plates and same pin withdrawal speed for all pins. Changes to pin withdrawal speed or volume in the source plate can result in different volumes being transferred.

Transfer volumes should always be confirmed by customers for their assay conditions and automated system.

Aqueous Solutions Pin Transfer Volumes Ranges

Aqueous Solutions on Uncoated Pins in 96 Format Microplates(1)
Pin TypePin Diameter(mm)Shape96 Format Low Range(nL)²96 Format High Range(nL)²
FP90.229Solid1339
FP80.356Solid1537
FP10.457Solid2261
FP1S60.4576nL Slot3467
FP1S100.45710nL Slot3974
FP1S500.45750nL Slot90124
FP30.787Solid93213
FP3S1000.787100nL Slot213334
FP3S2000.787200nL Slot311449
FP3S5000.787500nL Slot515671
FP40.914Solid126289
Footnotes: (1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well
Aqueous Solutions on Hydrophobic Pins in 96 Format Microplates(1)
Pin TypePin Diameter(mm)Shape96 Format Low Range(nL)²96 Format High Range(nL)²
FP90.229Solid1338
FP80.356Solid
FP10.457Solid2360
FP1S60.4576nL Slot3367
FP1S100.45710nL Slot4075
FP1S500.45750nL Slot86119
FP30.787Solid76209
FP3S1000.787100nL Slot188324
FP3S2000.787200nL Slot288436
FP3S5000.787500nL Slot473649
FP40.914Solid
Footnotes: (1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well
Aqueous Solution on E-Clip, Uncoated Pins(1)
Pin TypePin Diameter(mm)ShapeLow Range(nL)²High Range(nL)²
FP1.58Solid Pointed175594
FPS.51.58500nL Slot524962
FPS1.581000nL Slot10561476
FPS21.582000nL Slot17392174
FPS51.585000nL Slot51504953
FP61.58Solid Flat465960
FP6S.51.58500nL Slot9341445
FP6S1.581000nL Slot13961930
FP6S21.582000nL Slot20722637
FP6S51.585000nL Slot48204693
Footnotes:(1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well for 96 Format and 75ul source plate volume per well for 384 Format

DMSO Pin Transfer Volume Range Charts

Uncoated Pins in 96 and 384 Format Microplates(1)
Pin TypePin Diameter(mm)Shape96 Format Low Range(nL)²96 Format High Range(nL)²384 Format Low Range(nL)³384 Format High Range(nL)³
FP90.229Solid41038
FP80.35Solid1326618
FP10.457Solid18431131
FP1S60.4576nL Slot24491534
FP1S100.45710nL Slot30542140
FP1S200.45720nL Slot37612746
FP1S300.45730nL Slot46683554
FP1S400.45740nL Slot57784563
FP1S500.45750nL Slot70905675
FP30.787Solid671392979
FP40.91Solid941973498
FP3S1000.787100nL Slot175241114163
FP3S2000.787200nL Slot280332203250
FP3S5000.787500nL Slot535559427464
FP4S10000.911000nL Slot9401011704800
FP4S20000.912000nL Slot1518160812771362
Footnotes: (1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well (3) 75ul source plate volume per well
Hydrophobic-coated Pins in 96 and 384 Format Microplates(1)
Pin TypePin Diameter (mm)Shape96 Format Low Range(nL)²96 Format High Range(nL)²384 Format Low Range(nL)³384 Format High Range(nL)³
FP9H0.229Solid41038
FP8H0.35Solid924617
FP1H0.457Solid1539927
FP1S6H0.4576nL Slot23491432
FP1S10H0.45710nL Slot29532038
FP1S20H0.45720nL Slot35592643
FP1S30H0.45730nL Slot47693553
FP1S40H0.45740nL Slot54754158
FP1S50H0.45750nL Slot69905773
FP3H0.787Solid671342776
FP4H0.91Solid9518932102
FP3S100H0.787100nL Slot170227108164
FP3S200H0.787200nL Slot266320190239
FP3S500H0.787500nL Slot520542416456
FP4S1000H0.911000nL Slot9321000741805
FP4S2000H0.912000nL Slot1571163813511423
Footnotes: (1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well (3) 75ul source plate volume per well
E-Clip, Uncoated Pins, for 96 and 384 Format Microplates(1)
Pin TypeDiameter (mm)Shape96 Format Low Range(nL)²96 Format High Range(nL)²384 Format Low Range(nL)³384 Format High Range(nL)³
FP1.58Solid Pointed147411168395
FPS.51.58500nL Slot442704631843
FPS1.581000nL Slot893113013431498
FPS21.582000nL Slot1911203826072767
FPS51.585000nL Slot3908429651805253
FP61.58Solid Flat323674154398
FP6S.51.58500nL Slot73410428551053
FP6S1.581000nL Slot1210150016381717
FP6S21.582000nL Slot2299238427873068
FP6S51.585000nL Slot4329465652375245
Footnotes:(1) Delivery volume range is determined by speed of withdrawal from source liquid: Z-Speed Range = 1.5-30 mm/sec, slow speed = low volume delivery range, fast speed = high volume delivery range (2) 200ul source plate volume per well (3) 75ul source plate volume per well